Maturational growth of self-assembled, functional menisci as a result of TGF-β1 and enzymatic chondroitinase-ABC stimulation

Daniel J. Huey, Kyriacos A. Athanasiou

Research output: Contribution to journalArticle

60 Scopus citations

Abstract

Replacement of the knee meniscus requires a material possessing adequate geometrical and biomechanical properties. Meniscal tissue engineering attempts have been unable to produce tissue with collagen content and biomechanical properties, particularly tensile properties, mimicking native menisci. In an effort to obtain the geometric properties and the maturational growth necessary for the recapitulation of biochemical and, thus, biomechanical properties, a scaffoldless cell-based system, the self-assembly process, was used in conjunction with the catabolic enzyme chondroitinase-ABC and TGF-β1. We show that combinations of these agents resulted in maturational growth as evidenced by synergistic enhancement of the radial tensile modulus by 5-fold and the compressive relaxation modulus by 68%, and additive increases of the compressive instantaneous modulus by 136% and Col/WW by 196%. This study shows that tissue engineering can produce a biomaterial that is on par with the biochemical and biomechanical properties of native menisci.

Original languageEnglish (US)
Pages (from-to)2052-2058
Number of pages7
JournalBiomaterials
Volume32
Issue number8
DOIs
StatePublished - Mar 2011

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Keywords

  • C-ABC (chondroitinase-ABC)
  • Cartilage tissue engineering
  • ECM
  • Knee meniscus
  • Mechanical properties
  • TGF (transforming growth factor)

ASJC Scopus subject areas

  • Biomaterials
  • Bioengineering
  • Ceramics and Composites
  • Mechanics of Materials
  • Biophysics

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