Maturation of testicular tissue from infant monkeys after xenografting into mice

Rahul Rathi, Wenxian Zeng, Susan Megee, Alan J Conley, Stuart A Meyers, Ina Dobrinski

Research output: Contribution to journalArticle

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Abstract

In juvenile monkeys, precocious puberty can be induced by administration of gonadotropins resulting in testicular somatic cell maturation and germ cell differentiation. It is, however, unknown whether testicular maturation can also be induced in younger monkeys. Here we used testis tissue xenografting to investigate whether infant monkey testis tissue will undergo somatic cell maturation and/or spermatogenesis in response to endogenous adult mouse gonadotropins or exogenous gonadotropins. Testicular tissue pieces from 3- and 6-month-old rhesus monkeys were grafted to immunodeficient, castrated mice. Recipient mice were either left untreated or treated with pregnant mare serum gonadotropin and/or human chorionic gonadotropin twice weekly and were killed 28 weeks after grafting. Testicular maturation in grafted tissue was assessed based on morphology and the most advanced germ cell type present and by immunohistochemistry for expression of proliferating cell nuclear antigen, Mullerian-inhibiting substance, and androgen receptor. Testis grafts, irrespective of donor age or treatment, contained fewer germ cells than donor tissue. Grafts from 6-month-old donors showed tubular expansion with increased seminiferous tubule diameter and lumen formation, whereas those harvested from gonadotropin-treated mice contained elongated spermatids. Grafts from 3-month-old donors recovered from gonadotropin-treated mice contained pachytene spermatocytes, whereas those recovered from untreated mice showed only slight tubular expansion. Immunohistochemistry revealed that exposure to exogenous gonadotropins supported Sertoli cell maturation, irrespective of donor age. These results indicate that sustained gonadotropin stimulation of immature (<12 months old) monkey testis supports Sertoli cell maturation, thereby terminating the unresponsive phase of the germinal epithelium and allowing complete spermatogenesis in testis tissue from infant rhesus monkeys.

Original languageEnglish (US)
Pages (from-to)5288-5296
Number of pages9
JournalEndocrinology
Volume149
Issue number10
DOIs
StatePublished - Oct 2008

Fingerprint

Heterologous Transplantation
Gonadotropins
Haplorhini
Testis
Tissue Donors
Germ Cells
Sertoli Cells
Spermatogenesis
Macaca mulatta
Transplants
Immunohistochemistry
Equine Gonadotropins
Precocious Puberty
Seminiferous Tubules
Spermatocytes
Spermatids
Proliferating Cell Nuclear Antigen
Androgen Receptors
Chorionic Gonadotropin
Cell Differentiation

ASJC Scopus subject areas

  • Endocrinology

Cite this

Maturation of testicular tissue from infant monkeys after xenografting into mice. / Rathi, Rahul; Zeng, Wenxian; Megee, Susan; Conley, Alan J; Meyers, Stuart A; Dobrinski, Ina.

In: Endocrinology, Vol. 149, No. 10, 10.2008, p. 5288-5296.

Research output: Contribution to journalArticle

Rathi, Rahul ; Zeng, Wenxian ; Megee, Susan ; Conley, Alan J ; Meyers, Stuart A ; Dobrinski, Ina. / Maturation of testicular tissue from infant monkeys after xenografting into mice. In: Endocrinology. 2008 ; Vol. 149, No. 10. pp. 5288-5296.
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