Abstract
Matrix-assisted laser desorption/ionization (MALDI) was performed on individual, size-selected aerosol particles in the 2-8 μm diameter range. Monodisperse aerosol droplets containing matrix, analyte, and solvent were generated and entrained in a dry stream of air. The dried particles were drawn from atmospheric pressure directly into the source region of a reflecting field time-of-flight mass spectrometer. Individual particles were then detected by light scattering and analyzed on-the-fly by MALDI. The particle size and composition were systematically varied, and both liquid and solid matrices were studied. As the analyte-to-matrix mole ratio increased, the analyte signal intensity first increased and men leveled off. Quenching effects were observed when two different peptides were present in the same particle. These dependences were interpreted on the basis of analyte surface activity and adsorption isotherms. With a liquid matrix, the analyte is thought to partition between the particle surface and bulk. The signal intensity increases with analyte surface coverage until a monolayer is formed. With a solid matrix, the analyte is thought to adsorb on the surface of the original droplet containing matrix, analyte, and solvent When the solvent evaporates, the analyte deposits on the dry particle surface. Again, the signal intensity increases with analyte surface coverage until a monolayer is formed. With either a solid or liquid matrix, signal quenching is observed when multiple analytes compete for surface adsorption.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 3595-3601 |
| Number of pages | 7 |
| Journal | Analytical Chemistry |
| Volume | 68 |
| Issue number | 20 |
| State | Published - Oct 15 1996 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Analytical Chemistry
Cite this
Matrix-Assisted Laser Desorption/Ionization of Size- and Composition-Selected Aerosol Particles. / Mansoori, Bashir A.; Johnston, Murray V.; Wexler, Anthony S.
In: Analytical Chemistry, Vol. 68, No. 20, 15.10.1996, p. 3595-3601.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Matrix-Assisted Laser Desorption/Ionization of Size- and Composition-Selected Aerosol Particles
AU - Mansoori, Bashir A.
AU - Johnston, Murray V.
AU - Wexler, Anthony S.
PY - 1996/10/15
Y1 - 1996/10/15
N2 - Matrix-assisted laser desorption/ionization (MALDI) was performed on individual, size-selected aerosol particles in the 2-8 μm diameter range. Monodisperse aerosol droplets containing matrix, analyte, and solvent were generated and entrained in a dry stream of air. The dried particles were drawn from atmospheric pressure directly into the source region of a reflecting field time-of-flight mass spectrometer. Individual particles were then detected by light scattering and analyzed on-the-fly by MALDI. The particle size and composition were systematically varied, and both liquid and solid matrices were studied. As the analyte-to-matrix mole ratio increased, the analyte signal intensity first increased and men leveled off. Quenching effects were observed when two different peptides were present in the same particle. These dependences were interpreted on the basis of analyte surface activity and adsorption isotherms. With a liquid matrix, the analyte is thought to partition between the particle surface and bulk. The signal intensity increases with analyte surface coverage until a monolayer is formed. With a solid matrix, the analyte is thought to adsorb on the surface of the original droplet containing matrix, analyte, and solvent When the solvent evaporates, the analyte deposits on the dry particle surface. Again, the signal intensity increases with analyte surface coverage until a monolayer is formed. With either a solid or liquid matrix, signal quenching is observed when multiple analytes compete for surface adsorption.
AB - Matrix-assisted laser desorption/ionization (MALDI) was performed on individual, size-selected aerosol particles in the 2-8 μm diameter range. Monodisperse aerosol droplets containing matrix, analyte, and solvent were generated and entrained in a dry stream of air. The dried particles were drawn from atmospheric pressure directly into the source region of a reflecting field time-of-flight mass spectrometer. Individual particles were then detected by light scattering and analyzed on-the-fly by MALDI. The particle size and composition were systematically varied, and both liquid and solid matrices were studied. As the analyte-to-matrix mole ratio increased, the analyte signal intensity first increased and men leveled off. Quenching effects were observed when two different peptides were present in the same particle. These dependences were interpreted on the basis of analyte surface activity and adsorption isotherms. With a liquid matrix, the analyte is thought to partition between the particle surface and bulk. The signal intensity increases with analyte surface coverage until a monolayer is formed. With a solid matrix, the analyte is thought to adsorb on the surface of the original droplet containing matrix, analyte, and solvent When the solvent evaporates, the analyte deposits on the dry particle surface. Again, the signal intensity increases with analyte surface coverage until a monolayer is formed. With either a solid or liquid matrix, signal quenching is observed when multiple analytes compete for surface adsorption.
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UR - http://www.scopus.com/inward/citedby.url?scp=0030261908&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:0030261908
VL - 68
SP - 3595
EP - 3601
JO - Analytical Chemistry
JF - Analytical Chemistry
SN - 0003-2700
IS - 20
ER -