Maternal inheritance of mitochondrial DNA polymorphisms in cultured human fibroblasts

James Case, Douglas C. Wallace

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

We have isolated the total cellular DNA from the cultured diploid fibroblasts of a six-member, three-generation human family. Using a specific radioactive probe for mitochondrial (mt) sequences we have identified new polymorphic variants in this family for the Hhal restriction endonuclease cleavage pattern of the mtDNA. The inheritance of these cleavage patterns verifies the maternal inheritance of mtDNA through all three generations.

Original languageEnglish (US)
Pages (from-to)103-108
Number of pages6
JournalSomatic Cell Genetics
Volume7
Issue number1
DOIs
StatePublished - Jan 1 1981

Fingerprint

Mitochondrial DNA
Fibroblasts
Inheritance Patterns
DNA Restriction Enzymes
Diploidy
DNA
Maternal Inheritance

ASJC Scopus subject areas

  • Medicine(all)
  • Genetics

Cite this

Maternal inheritance of mitochondrial DNA polymorphisms in cultured human fibroblasts. / Case, James; Wallace, Douglas C.

In: Somatic Cell Genetics, Vol. 7, No. 1, 01.01.1981, p. 103-108.

Research output: Contribution to journalArticle

@article{3d532aeabd8e4902a461c4ed5a35a1cd,
title = "Maternal inheritance of mitochondrial DNA polymorphisms in cultured human fibroblasts",
abstract = "We have isolated the total cellular DNA from the cultured diploid fibroblasts of a six-member, three-generation human family. Using a specific radioactive probe for mitochondrial (mt) sequences we have identified new polymorphic variants in this family for the Hhal restriction endonuclease cleavage pattern of the mtDNA. The inheritance of these cleavage patterns verifies the maternal inheritance of mtDNA through all three generations.",
author = "James Case and Wallace, {Douglas C.}",
year = "1981",
month = "1",
day = "1",
doi = "10.1007/BF01544751",
language = "English (US)",
volume = "7",
pages = "103--108",
journal = "Somatic Cell and Molecular Genetics",
issn = "0740-7750",
publisher = "Springer GmbH & Co, Auslieferungs-Gesellschaf",
number = "1",

}

TY - JOUR

T1 - Maternal inheritance of mitochondrial DNA polymorphisms in cultured human fibroblasts

AU - Case, James

AU - Wallace, Douglas C.

PY - 1981/1/1

Y1 - 1981/1/1

N2 - We have isolated the total cellular DNA from the cultured diploid fibroblasts of a six-member, three-generation human family. Using a specific radioactive probe for mitochondrial (mt) sequences we have identified new polymorphic variants in this family for the Hhal restriction endonuclease cleavage pattern of the mtDNA. The inheritance of these cleavage patterns verifies the maternal inheritance of mtDNA through all three generations.

AB - We have isolated the total cellular DNA from the cultured diploid fibroblasts of a six-member, three-generation human family. Using a specific radioactive probe for mitochondrial (mt) sequences we have identified new polymorphic variants in this family for the Hhal restriction endonuclease cleavage pattern of the mtDNA. The inheritance of these cleavage patterns verifies the maternal inheritance of mtDNA through all three generations.

UR - http://www.scopus.com/inward/record.url?scp=0019429346&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019429346&partnerID=8YFLogxK

U2 - 10.1007/BF01544751

DO - 10.1007/BF01544751

M3 - Article

C2 - 6261411

AN - SCOPUS:0019429346

VL - 7

SP - 103

EP - 108

JO - Somatic Cell and Molecular Genetics

JF - Somatic Cell and Molecular Genetics

SN - 0740-7750

IS - 1

ER -