Maternal high-protein or high-prebiotic-fiber diets affect maternal milk composition and gut microbiota in rat dams and their offspring

Megan C. Hallam, Daniela Barile, Mickael Meyrand, J. Bruce German, Raylene A. Reimer

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Objective: Maternal gut microbiota and milk composition could modify offspring microbiota and therefore disease susceptibility. The effect of maternal high-protein (HP) or prebiotic diets on maternal milk composition and gut microbiota in rat dams and offspring was examined. Methods: Wistar rat dams were fed a control, HP (40% wt/wt), or high-prebiotic-fiber (21.6% wt/wt) (HF) diet throughout pregnancy and lactation. Pups were challenged with a high-fat/sucrose diet from 14.5 to 22.5 weeks of age. Dam milk was analyzed for fat, protein, and oligosaccharides (OS). Fecal microbiota was analyzed in dams at parturition and 2 weeks post-partum and in offspring at 5 and 22 weeks along with cecal digesta at termination. Results: Maternal milk differed only in OS content, each diet group being distinguishable. HF1 and HP1 offspring had decreased plasma lipopolysaccharide compared with C1. Offspring sex, maternal diet, and time (5 weeks vs. 22 weeks of age) affected the microbial groups examined. Bifidobacteria was higher in HF dams and offspring. Conclusions: Increasing protein or fiber content in maternal diet during pregnancy and lactation modifies milk OS content and gut microbiota of dams which may influence establishment of gut microbiota in offspring.

Original languageEnglish (US)
Pages (from-to)2344-2351
Number of pages8
JournalObesity
Volume22
Issue number11
DOIs
StatePublished - Nov 1 2014
Externally publishedYes

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Prebiotics
Milk
Mothers
Diet
Oligosaccharides
Proteins
Microbiota
Lactation
Pregnancy
Bifidobacterium
Disease Susceptibility
High Fat Diet
Lipopolysaccharides
Sucrose
Gastrointestinal Microbiome
Wistar Rats
Fats
Parturition

ASJC Scopus subject areas

  • Endocrinology
  • Medicine (miscellaneous)
  • Endocrinology, Diabetes and Metabolism
  • Nutrition and Dietetics

Cite this

Maternal high-protein or high-prebiotic-fiber diets affect maternal milk composition and gut microbiota in rat dams and their offspring. / Hallam, Megan C.; Barile, Daniela; Meyrand, Mickael; Bruce German, J.; Reimer, Raylene A.

In: Obesity, Vol. 22, No. 11, 01.11.2014, p. 2344-2351.

Research output: Contribution to journalArticle

Hallam, Megan C. ; Barile, Daniela ; Meyrand, Mickael ; Bruce German, J. ; Reimer, Raylene A. / Maternal high-protein or high-prebiotic-fiber diets affect maternal milk composition and gut microbiota in rat dams and their offspring. In: Obesity. 2014 ; Vol. 22, No. 11. pp. 2344-2351.
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