Mass transfer in amperometric biosensors based on nanocomposite thin films of redox polymers and oxidoreductases

Michael V. Pishko, Alexander Revzin, Aleksandr L. Simonian

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Mass transfer in nanocomposite hydrogel thin films consisting of alternating layers of an organometallic redox polymer (RP) and oxidoreductase enzymes was investigated. Multilayer nanostructures were fabricated on gold surfaces by the deposition of an anionic self-assembled monolayer of 11-mercaptoundecanoic acid, followed by the electrostatic binding of a cationic redox polymer, poly[vinylpyridine Os(bis-bipyridine)2Cl-co- allylamine], and an anionic oxidoreductase. Surface plasmon resonance spectroscopy, Fourier transform infrared external reflection spectroscopy (FTIR-ERS), ellipsometry and electrochemistry were employed to characterize the assembly of these nanocomposite films. Simultaneous SPR/electrochemistry enabled real time observation of the assembly of sensing components, changes in film structure with electrode potential, and the immediate, in situ electrochemical verification of substrate-dependent current upon the addition of enzyme to the multilayer structure. SPR and FTIR-ERS studies also showed no desorption of polymer or enzyme from the nanocomposite structure when stored in aqueous environment occurred over the period of three weeks, suggesting that decreasing in substrate sensitivity were due to loss of enzymatic activity rather than loss of film compounds from the nanostructure.

Original languageEnglish (US)
Pages (from-to)79-90
Number of pages12
JournalSensors
Volume2
Issue number3
StatePublished - 2002
Externally publishedYes

Fingerprint

Nanocomposites
Nanocomposite films
Biosensing Techniques
bioinstrumentation
Biosensors
Oxidation-Reduction
mass transfer
Electrochemistry
enzymes
nanocomposites
Oxidoreductases
Polymers
Mass transfer
Enzymes
Nanostructures
electrochemistry
Thin films
Spectrum Analysis
Multilayers
polymers

Keywords

  • Biosensor
  • Enzyme
  • Hydrogel
  • Nanocomposite
  • Redox

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry

Cite this

Mass transfer in amperometric biosensors based on nanocomposite thin films of redox polymers and oxidoreductases. / Pishko, Michael V.; Revzin, Alexander; Simonian, Aleksandr L.

In: Sensors, Vol. 2, No. 3, 2002, p. 79-90.

Research output: Contribution to journalArticle

Pishko, Michael V. ; Revzin, Alexander ; Simonian, Aleksandr L. / Mass transfer in amperometric biosensors based on nanocomposite thin films of redox polymers and oxidoreductases. In: Sensors. 2002 ; Vol. 2, No. 3. pp. 79-90.
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