Mass spectrometry imaging reveals heterogeneous efavirenz distribution within putative HIV reservoirs

Corbin G. Thompson, Mark T. Bokhart, Craig Sykes, Lourdes Adamson, Yuri Fedoriw, Paul A Luciw, David C. Muddiman, Angela D M Kashuba, Elias P. Rosen

Research output: Contribution to journalArticle

31 Scopus citations

Abstract

Persistent HIV replication within active viral reservoirs may be caused by inadequate antiretroviral penetration. Here, we used mass spectrometry imaging with infrared matrix-assisted laser desorption-electrospray ionization to quantify the distribution of efavirenz within tissues from a macaque dosed orally to a steady state. Intratissue efavirenz distribution was heterogeneous, with the drug concentrating in the lamina propria of the colon, the primary follicles of lymph nodes, and the brain gray matter. These are the first imaging data of an antiretroviral drug in active viral reservoirs.

Original languageEnglish (US)
Pages (from-to)2944-2948
Number of pages5
JournalAntimicrobial Agents and Chemotherapy
Volume59
Issue number5
DOIs
StatePublished - May 1 2015

ASJC Scopus subject areas

  • Pharmacology (medical)
  • Pharmacology
  • Infectious Diseases

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    Thompson, C. G., Bokhart, M. T., Sykes, C., Adamson, L., Fedoriw, Y., Luciw, P. A., Muddiman, D. C., Kashuba, A. D. M., & Rosen, E. P. (2015). Mass spectrometry imaging reveals heterogeneous efavirenz distribution within putative HIV reservoirs. Antimicrobial Agents and Chemotherapy, 59(5), 2944-2948. https://doi.org/10.1128/AAC.04952-14