Mass-spectrometry-based metabolomics

Limitations and recommendations for future progress with particular focus on nutrition research

Augustin Scalbert, Lorraine Brennan, Oliver Fiehn, Thomas Hankemeier, Bruce S. Kristal, Ben van Ommen, Estelle Pujos-Guillot, Elwin Verheij, David Wishart, Suzan Wopereis

Research output: Contribution to journalArticle

329 Citations (Scopus)

Abstract

Mass spectrometry (MS) techniques, because of their sensitivity and selectivity, have become methods of choice to characterize the human metabolome and MS-based metabolomics is increasingly used to characterize the complex metabolic effects of nutrients or foods. However progress is still hampered by many unsolved problems and most notably the lack of well established and standardized methods or procedures, and the difficulties still met in the identification of the metabolites influenced by a given nutritional intervention. The purpose of this paper is to review the main obstacles limiting progress and to make recommendations to overcome them. Propositions are made to improve the mode of collection and preparation of biological samples, the coverage and quality of mass spectrometry analyses, the extraction and exploitation of the raw data, the identification of the metabolites and the biological interpretation of the results.

Original languageEnglish (US)
Pages (from-to)435-458
Number of pages24
JournalMetabolomics
Volume5
Issue number4
DOIs
StatePublished - Dec 2009

Fingerprint

Metabolomics
Nutrition
Mass spectrometry
Mass Spectrometry
Metabolites
Research
Food
Metabolome
Nutrients

Keywords

  • Mass spectrometry
  • Metabolomics
  • Method development
  • Nutrition

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Endocrinology, Diabetes and Metabolism

Cite this

Scalbert, A., Brennan, L., Fiehn, O., Hankemeier, T., Kristal, B. S., van Ommen, B., ... Wopereis, S. (2009). Mass-spectrometry-based metabolomics: Limitations and recommendations for future progress with particular focus on nutrition research. Metabolomics, 5(4), 435-458. https://doi.org/10.1007/s11306-009-0168-0

Mass-spectrometry-based metabolomics : Limitations and recommendations for future progress with particular focus on nutrition research. / Scalbert, Augustin; Brennan, Lorraine; Fiehn, Oliver; Hankemeier, Thomas; Kristal, Bruce S.; van Ommen, Ben; Pujos-Guillot, Estelle; Verheij, Elwin; Wishart, David; Wopereis, Suzan.

In: Metabolomics, Vol. 5, No. 4, 12.2009, p. 435-458.

Research output: Contribution to journalArticle

Scalbert, A, Brennan, L, Fiehn, O, Hankemeier, T, Kristal, BS, van Ommen, B, Pujos-Guillot, E, Verheij, E, Wishart, D & Wopereis, S 2009, 'Mass-spectrometry-based metabolomics: Limitations and recommendations for future progress with particular focus on nutrition research', Metabolomics, vol. 5, no. 4, pp. 435-458. https://doi.org/10.1007/s11306-009-0168-0
Scalbert, Augustin ; Brennan, Lorraine ; Fiehn, Oliver ; Hankemeier, Thomas ; Kristal, Bruce S. ; van Ommen, Ben ; Pujos-Guillot, Estelle ; Verheij, Elwin ; Wishart, David ; Wopereis, Suzan. / Mass-spectrometry-based metabolomics : Limitations and recommendations for future progress with particular focus on nutrition research. In: Metabolomics. 2009 ; Vol. 5, No. 4. pp. 435-458.
@article{17377235045a48bd9c6e9ef394f4c133,
title = "Mass-spectrometry-based metabolomics: Limitations and recommendations for future progress with particular focus on nutrition research",
abstract = "Mass spectrometry (MS) techniques, because of their sensitivity and selectivity, have become methods of choice to characterize the human metabolome and MS-based metabolomics is increasingly used to characterize the complex metabolic effects of nutrients or foods. However progress is still hampered by many unsolved problems and most notably the lack of well established and standardized methods or procedures, and the difficulties still met in the identification of the metabolites influenced by a given nutritional intervention. The purpose of this paper is to review the main obstacles limiting progress and to make recommendations to overcome them. Propositions are made to improve the mode of collection and preparation of biological samples, the coverage and quality of mass spectrometry analyses, the extraction and exploitation of the raw data, the identification of the metabolites and the biological interpretation of the results.",
keywords = "Mass spectrometry, Metabolomics, Method development, Nutrition",
author = "Augustin Scalbert and Lorraine Brennan and Oliver Fiehn and Thomas Hankemeier and Kristal, {Bruce S.} and {van Ommen}, Ben and Estelle Pujos-Guillot and Elwin Verheij and David Wishart and Suzan Wopereis",
year = "2009",
month = "12",
doi = "10.1007/s11306-009-0168-0",
language = "English (US)",
volume = "5",
pages = "435--458",
journal = "Metabolomics",
issn = "1573-3882",
publisher = "Springer New York",
number = "4",

}

TY - JOUR

T1 - Mass-spectrometry-based metabolomics

T2 - Limitations and recommendations for future progress with particular focus on nutrition research

AU - Scalbert, Augustin

AU - Brennan, Lorraine

AU - Fiehn, Oliver

AU - Hankemeier, Thomas

AU - Kristal, Bruce S.

AU - van Ommen, Ben

AU - Pujos-Guillot, Estelle

AU - Verheij, Elwin

AU - Wishart, David

AU - Wopereis, Suzan

PY - 2009/12

Y1 - 2009/12

N2 - Mass spectrometry (MS) techniques, because of their sensitivity and selectivity, have become methods of choice to characterize the human metabolome and MS-based metabolomics is increasingly used to characterize the complex metabolic effects of nutrients or foods. However progress is still hampered by many unsolved problems and most notably the lack of well established and standardized methods or procedures, and the difficulties still met in the identification of the metabolites influenced by a given nutritional intervention. The purpose of this paper is to review the main obstacles limiting progress and to make recommendations to overcome them. Propositions are made to improve the mode of collection and preparation of biological samples, the coverage and quality of mass spectrometry analyses, the extraction and exploitation of the raw data, the identification of the metabolites and the biological interpretation of the results.

AB - Mass spectrometry (MS) techniques, because of their sensitivity and selectivity, have become methods of choice to characterize the human metabolome and MS-based metabolomics is increasingly used to characterize the complex metabolic effects of nutrients or foods. However progress is still hampered by many unsolved problems and most notably the lack of well established and standardized methods or procedures, and the difficulties still met in the identification of the metabolites influenced by a given nutritional intervention. The purpose of this paper is to review the main obstacles limiting progress and to make recommendations to overcome them. Propositions are made to improve the mode of collection and preparation of biological samples, the coverage and quality of mass spectrometry analyses, the extraction and exploitation of the raw data, the identification of the metabolites and the biological interpretation of the results.

KW - Mass spectrometry

KW - Metabolomics

KW - Method development

KW - Nutrition

UR - http://www.scopus.com/inward/record.url?scp=74449085466&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=74449085466&partnerID=8YFLogxK

U2 - 10.1007/s11306-009-0168-0

DO - 10.1007/s11306-009-0168-0

M3 - Article

VL - 5

SP - 435

EP - 458

JO - Metabolomics

JF - Metabolomics

SN - 1573-3882

IS - 4

ER -