Mass spectrometry-based metabolomics

Katja Dettmer, Pavel A. Aronov, Bruce D. Hammock

Research output: Contribution to journalArticle

1125 Citations (Scopus)

Abstract

This review presents an overview of the dynamically developing field of mass spectrometry-based metabolomics. Metabolomics aims at the comprehensive and quantitative analysis of wide arrays of metabolites in biological samples. These numerous analytes have very diverse physico-chemical properties and occur at different abundance levels. Consequently, comprehensive metabolomics investigations are primarily a challenge for analytical chemistry and specifically mass spectrometry has vast potential as a tool for this type of investigation. Metabolomics require special approaches for sample preparation, separation, and mass spectrometric analysis. Current examples of those approaches are described in this review. It primarily focuses on metabolic fingerprinting, a technique that analyzes all detectable analytes in a given sample with subsequent classification of samples and identification of differentially expressed metabolites, which define the sample classes. To perform this complex task, data analysis tools, metabolite libraries, and databases are required. Therefore, recent advances in metabolomics bioinformatics are also discussed.

Original languageEnglish (US)
Pages (from-to)51-78
Number of pages28
JournalMass Spectrometry Reviews
Volume26
Issue number1
DOIs
StatePublished - Jan 2007

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Metabolomics
Mass spectrometry
Mass Spectrometry
mass spectroscopy
metabolites
Metabolites
analytical chemistry
Bioinformatics
Computational Biology
Chemical analysis
chemical properties
Chemical properties
quantitative analysis
Libraries
Databases
preparation

Keywords

  • Lipidomics
  • Mass spectrometry
  • Metabolic fingerprinting
  • Metabolic profiling
  • Metabolomics

ASJC Scopus subject areas

  • Spectroscopy

Cite this

Dettmer, K., Aronov, P. A., & Hammock, B. D. (2007). Mass spectrometry-based metabolomics. Mass Spectrometry Reviews, 26(1), 51-78. https://doi.org/10.1002/mas.20108

Mass spectrometry-based metabolomics. / Dettmer, Katja; Aronov, Pavel A.; Hammock, Bruce D.

In: Mass Spectrometry Reviews, Vol. 26, No. 1, 01.2007, p. 51-78.

Research output: Contribution to journalArticle

Dettmer, K, Aronov, PA & Hammock, BD 2007, 'Mass spectrometry-based metabolomics', Mass Spectrometry Reviews, vol. 26, no. 1, pp. 51-78. https://doi.org/10.1002/mas.20108
Dettmer, Katja ; Aronov, Pavel A. ; Hammock, Bruce D. / Mass spectrometry-based metabolomics. In: Mass Spectrometry Reviews. 2007 ; Vol. 26, No. 1. pp. 51-78.
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