Mass Spectrometry Approaches to Glycomic and Glycoproteomic Analyses

L. Renee Ruhaak, Gege Xu, Qiongyu Li, Elisha Goonatilleke, Carlito B Lebrilla

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Glycomic and glycoproteomic analyses involve the characterization of oligosaccharides (glycans) conjugated to proteins. Glycans are produced through a complicated nontemplate driven process involving the competition of enzymes that extend the nascent chain. The large diversity of structures, the variations in polarity of the individual saccharide residues, and the poor ionization efficiencies of glycans all conspire to make the analysis arguably much more difficult than any other biopolymer. Furthermore, the large number of glycoforms associated with a specific protein site makes it more difficult to characterize than any post-translational modification. Nonetheless, there have been significant progress, and advanced separation and mass spectrometry methods have been at its center and the main reason for the progress. While glycomic and glycoproteomic analyses are still typically available only through highly specialized laboratories, new software and workflow is making it more accessible. This review focuses on the role of mass spectrometry and separation methods in advancing glycomic and glycoproteomic analyses. It describes the current state of the field and progress toward making it more available to the larger scientific community.

Original languageEnglish (US)
Pages (from-to)7886-7930
Number of pages45
JournalChemical Reviews
Volume118
Issue number17
DOIs
StatePublished - Sep 12 2018

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Mass spectrometry
Polysaccharides
Biopolymers
Oligosaccharides
Ionization
Proteins
Enzymes
Glycomics

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Ruhaak, L. R., Xu, G., Li, Q., Goonatilleke, E., & Lebrilla, C. B. (2018). Mass Spectrometry Approaches to Glycomic and Glycoproteomic Analyses. Chemical Reviews, 118(17), 7886-7930. https://doi.org/10.1021/acs.chemrev.7b00732

Mass Spectrometry Approaches to Glycomic and Glycoproteomic Analyses. / Ruhaak, L. Renee; Xu, Gege; Li, Qiongyu; Goonatilleke, Elisha; Lebrilla, Carlito B.

In: Chemical Reviews, Vol. 118, No. 17, 12.09.2018, p. 7886-7930.

Research output: Contribution to journalArticle

Ruhaak, LR, Xu, G, Li, Q, Goonatilleke, E & Lebrilla, CB 2018, 'Mass Spectrometry Approaches to Glycomic and Glycoproteomic Analyses', Chemical Reviews, vol. 118, no. 17, pp. 7886-7930. https://doi.org/10.1021/acs.chemrev.7b00732
Ruhaak LR, Xu G, Li Q, Goonatilleke E, Lebrilla CB. Mass Spectrometry Approaches to Glycomic and Glycoproteomic Analyses. Chemical Reviews. 2018 Sep 12;118(17):7886-7930. https://doi.org/10.1021/acs.chemrev.7b00732
Ruhaak, L. Renee ; Xu, Gege ; Li, Qiongyu ; Goonatilleke, Elisha ; Lebrilla, Carlito B. / Mass Spectrometry Approaches to Glycomic and Glycoproteomic Analyses. In: Chemical Reviews. 2018 ; Vol. 118, No. 17. pp. 7886-7930.
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