Mass spectrometric methods for analysis of oligosaccharides in human milk

Milady R. Ninonuevo, Carlito B Lebrilla

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

Establishing the analytical platforms for characterizing human milk oligosaccharides is important to fully assess their specific functionalities. The characterization of these biomolecules, however, is still considered challenging, owing to their overall complexity and diversity. Addressed here are the technical difficulties with an emphasis on the application of mass spectrometry to rapidly profile and quantify human milk oligosaccharides. Fundamental concepts and improvements in instrumentation and an overview of the biological functions and structures of these compounds are also discussed. Results reveal that small-chain oligosaccharides, evident in abundance in the early stage of lactation, are selectively consumed by specific stains of Bifidobacterium longum biovar, infantis.

Original languageEnglish (US)
JournalNutrition Reviews
Volume67
Issue numberSUPPL. 2
DOIs
StatePublished - Nov 2009

Fingerprint

Human Milk
Oligosaccharides
Lactation
Mass Spectrometry
Coloring Agents

Keywords

  • Bifidobacteria
  • FTICR-MS
  • HPLC-Chip/TOF-MS
  • Human milk oligosaccharides
  • Microfluidic

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

Mass spectrometric methods for analysis of oligosaccharides in human milk. / Ninonuevo, Milady R.; Lebrilla, Carlito B.

In: Nutrition Reviews, Vol. 67, No. SUPPL. 2, 11.2009.

Research output: Contribution to journalArticle

Ninonuevo, Milady R. ; Lebrilla, Carlito B. / Mass spectrometric methods for analysis of oligosaccharides in human milk. In: Nutrition Reviews. 2009 ; Vol. 67, No. SUPPL. 2.
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