Mass cytometry reveals a distinct immunoprofile of operational tolerance in pediatric liver transplantation

Audrey H. Lau, Matthew J. Vitalone, Kelly Haas, Todd Shawler, Carlos O. Esquivel, William E. Berquist, Olivia M. Martinez, Ricardo O. Castillo, Sheri M. Krams

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

Long-term IS in transplant patients has significant morbidity, poorer quality of life, and substantial economic costs. TOL, defined as graft acceptance without functional impairment in the absence of IS, has been achieved in some pediatric LT recipients. Using mass cytometry, peripheral blood immunotyping was performed to characterize differences between tolerant patients and patients who are stable on single-agent IS. Single-cell mass cytometry was performed using blood samples from a single-center pediatric LT population of operationally tolerant patients to comprehensively characterize the immune cell populations in the tolerant state compared with patients on chronic low-dose IS. Specific T-cell populations of interest were confirmed by flow cytometry. This high-dimensional phenotypic analysis revealed distinct immunoprofiles between transplant populations as well as a CD4+ TOT (CD4+CD5+CD25+CD38−/loCD45RA) that correlates with tolerance in pediatric LT recipients. In TOL patients, the TOT was significantly increased as compared to patients stable on low levels of IS. This TOT cell was confirmed by flow cytometry and is distinct from classic Treg cells. These results demonstrate the power of mass cytometry to discover significant immune cell signatures that have diagnostic potential.

Original languageEnglish (US)
Pages (from-to)1072-1080
Number of pages9
JournalPediatric Transplantation
Volume20
Issue number8
DOIs
StatePublished - Dec 1 2016
Externally publishedYes

Keywords

  • cytometry by time of flight
  • immune cells
  • peripheral blood mononuclear cells
  • post-transplant
  • T cells

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Transplantation

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  • Cite this

    Lau, A. H., Vitalone, M. J., Haas, K., Shawler, T., Esquivel, C. O., Berquist, W. E., Martinez, O. M., Castillo, R. O., & Krams, S. M. (2016). Mass cytometry reveals a distinct immunoprofile of operational tolerance in pediatric liver transplantation. Pediatric Transplantation, 20(8), 1072-1080. https://doi.org/10.1111/petr.12795