Abstract
Recommendations for vitamin A intake and liver stores are based on maintaining normal vision. We propose that higher levels may be required to maintain normal innate immune function. To test this hypothesis, we conducted an 8-wk residential study among 36 healthy Bangladeshi men with low vitamin A stores. Subjects were randomized to receive vitamin A (240 mg in 4 doses) or placebo during study wk 2 and 3. They received 2 vaccines during wk 5 and vitamin A stores were estimated by isotopic dilution at wk 8. The serum concentration of the chemokine interferon-γ-induced protein 10, a component of T-helper 1 (Th1) response, increased significantly after supplementation and was positively and significantly associated with vitamin A stores. Blood concentrations of natural killer (NK) and NK T-cells, which have anticancer and antiviral activity, were positively associated with stores (P < 0.05), as was monocyte oxidative burst (P < 0.05), a marker of bacterial killing ability. However, serum interleukin (IL)-6 and IL-17, cytokines that regulate the antibacterial Th 17 response, were significantly and negatively associated with stores, as was production of the regulatory cytokine IL-10 by whole-blood cultures stimulated with bacterial lipopolysaccharide. In summary, vitamin A stores were positively associated with several measures of innate immune activity across a broad range of stores, suggesting that vitamin A enhances protection against diverse pathogens even at concentrations above those needed to maintain normal vision. The negative association of stores with serum IL-6 and IL-17 suggests that not all protective responses are similarly enhanced by vitamin A.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 377-385 |
| Number of pages | 9 |
| Journal | Journal of Nutrition |
| Volume | 139 |
| Issue number | 2 |
| DOIs | |
| State | Published - Feb 2009 |
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ASJC Scopus subject areas
- Medicine (miscellaneous)
- Nutrition and Dietetics
- Medicine(all)
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Markers of innate immune function are associated with vitamin A stores in men. / Ahmad, Shaikh M.; Haskell, Marjorie J.; Raqib, Rubhana; Stephensen, Charles B.
In: Journal of Nutrition, Vol. 139, No. 2, 02.2009, p. 377-385.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Markers of innate immune function are associated with vitamin A stores in men
AU - Ahmad, Shaikh M.
AU - Haskell, Marjorie J.
AU - Raqib, Rubhana
AU - Stephensen, Charles B.
PY - 2009/2
Y1 - 2009/2
N2 - Recommendations for vitamin A intake and liver stores are based on maintaining normal vision. We propose that higher levels may be required to maintain normal innate immune function. To test this hypothesis, we conducted an 8-wk residential study among 36 healthy Bangladeshi men with low vitamin A stores. Subjects were randomized to receive vitamin A (240 mg in 4 doses) or placebo during study wk 2 and 3. They received 2 vaccines during wk 5 and vitamin A stores were estimated by isotopic dilution at wk 8. The serum concentration of the chemokine interferon-γ-induced protein 10, a component of T-helper 1 (Th1) response, increased significantly after supplementation and was positively and significantly associated with vitamin A stores. Blood concentrations of natural killer (NK) and NK T-cells, which have anticancer and antiviral activity, were positively associated with stores (P < 0.05), as was monocyte oxidative burst (P < 0.05), a marker of bacterial killing ability. However, serum interleukin (IL)-6 and IL-17, cytokines that regulate the antibacterial Th 17 response, were significantly and negatively associated with stores, as was production of the regulatory cytokine IL-10 by whole-blood cultures stimulated with bacterial lipopolysaccharide. In summary, vitamin A stores were positively associated with several measures of innate immune activity across a broad range of stores, suggesting that vitamin A enhances protection against diverse pathogens even at concentrations above those needed to maintain normal vision. The negative association of stores with serum IL-6 and IL-17 suggests that not all protective responses are similarly enhanced by vitamin A.
AB - Recommendations for vitamin A intake and liver stores are based on maintaining normal vision. We propose that higher levels may be required to maintain normal innate immune function. To test this hypothesis, we conducted an 8-wk residential study among 36 healthy Bangladeshi men with low vitamin A stores. Subjects were randomized to receive vitamin A (240 mg in 4 doses) or placebo during study wk 2 and 3. They received 2 vaccines during wk 5 and vitamin A stores were estimated by isotopic dilution at wk 8. The serum concentration of the chemokine interferon-γ-induced protein 10, a component of T-helper 1 (Th1) response, increased significantly after supplementation and was positively and significantly associated with vitamin A stores. Blood concentrations of natural killer (NK) and NK T-cells, which have anticancer and antiviral activity, were positively associated with stores (P < 0.05), as was monocyte oxidative burst (P < 0.05), a marker of bacterial killing ability. However, serum interleukin (IL)-6 and IL-17, cytokines that regulate the antibacterial Th 17 response, were significantly and negatively associated with stores, as was production of the regulatory cytokine IL-10 by whole-blood cultures stimulated with bacterial lipopolysaccharide. In summary, vitamin A stores were positively associated with several measures of innate immune activity across a broad range of stores, suggesting that vitamin A enhances protection against diverse pathogens even at concentrations above those needed to maintain normal vision. The negative association of stores with serum IL-6 and IL-17 suggests that not all protective responses are similarly enhanced by vitamin A.
UR - http://www.scopus.com/inward/record.url?scp=60249090145&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=60249090145&partnerID=8YFLogxK
U2 - 10.3945/jn.108.100198
DO - 10.3945/jn.108.100198
M3 - Article
C2 - 19091796
AN - SCOPUS:60249090145
VL - 139
SP - 377
EP - 385
JO - Journal of Nutrition
JF - Journal of Nutrition
SN - 0022-3166
IS - 2
ER -