Marine and Anthropogenic Bromopyrroles Alter Cellular Ca2+Dynamics of Murine Cortical Neuronal Networks by Targeting the Ryanodine Receptor and Sarco/Endoplasmic Reticulum Ca2+-ATPase

Jing Zheng, Shane Antrobus, Wei Feng, Trevor N. Purdy, Bradley S. Moore, Isaac N. Pessah

Research output: Contribution to journalArticlepeer-review

Abstract

Bromopyrroles (BrPyr) are synthesized naturally by marine sponge symbionts and produced anthropogenically as byproducts of wastewater treatment. BrPyr interact with ryanodine receptors (RYRs) and sarco/endoplasmic reticulum (SR/ER) Ca2+-ATPase (SERCA). Influences of BrPyr on the neuronal network activity remain uncharted. BrPyr analogues with differing spectra of RYR/SERCA activities were tested using RYR-null or RYR1-expressing HEK293 and murine cortical neuronal/glial cocultures (NGCs) loaded with Fluo-4 to elucidate their mechanisms altering Ca2+dynamics. The NGC electrical spike activity (ESA) was measured from NGCs plated on multielectrode arrays. Nanomolar tetrabromopyrrole (TBP,1) potentiated caffeine-triggered Ca2+release independent of extracellular [Ca2+] in RYR1-HEK293, whereas higher concentrations produce slow and sustained rise in cytoplasmic [Ca2+] independent of RYR1 expression. TBP, 2,3,5-tribromopyrrole (2), pyrrole (3), 2,3,4-tribromopyrrole (4), and ethyl 4-bromopyrrole-2-carboxylate (5) added acutely to NGC showed differential potency; rank orderTBP(IC50≈ 220 nM) >2≫5, whereas3and4were inactive at 10 μM.TBP>2 μM elicited sustained elevation of cytoplasmic [Ca2+] and loss of neuronal viability.TBPdid not alter network ESA. BrPyr from marine and anthropogenic sources are ecological signaling molecules and emerging anthropogenic pollutants of concern to environmental and human health that potently alter ER Ca2+dynamics and warrant further investigationin vivo.

Original languageEnglish (US)
Pages (from-to)16023-16033
Number of pages11
JournalEnvironmental Science and Technology
Volume55
Issue number23
DOIs
StatePublished - Dec 7 2021
Externally publishedYes

Keywords

  • bromopyrroles
  • Ca2+ homeostasis
  • disinfectant byproducts
  • marine organohalogens
  • neurotoxicity
  • ryanodine receptors
  • sarco/endoplasmic reticulum Ca2+-ATPase (SERCA)
  • structure−activity relationship

ASJC Scopus subject areas

  • Chemistry(all)
  • Environmental Chemistry

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