Mapping the 3D connectivity of the rat inner retinal vascular network using OCT angiography

Conor Leahy, Harsha Radhakrishnan, Geoffrey Weiner, Jeffrey L. Goldberg, Vivek Srinivasan

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

PURPOSE. The purpose of this study is to demonstrate three-dimensional (3D) graphing based on optical coherence tomography (OCT) angiography for characterization of the inner retinal vascular architecture and determination of its topologic principles. METHODS. Rat eyes (N = 3) were imaged with a 1300-nm spectral/Fourier domain OCT microscope. A topologic model of the inner retinal vascular network was obtained from OCT angiography data using a combination of automated and manually-guided image processing techniques. Using a resistive network model, with experimentally-quantified flow in major retinal vessels near the optic nerve head as boundary conditions, theoretical changes in the distribution of flow induced by vessel dilations were inferred. RESULTS. A topologically-representative 3D vectorized graph of the inner retinal vasculature, derived from OCT angiography data, is presented. The laminar and compartmental connectivity of the vasculature are characterized. In contrast to sparse connectivity between the superficial vitreal vasculature and capillary plexuses of the inner retina, connectivity between the two capillary plexus layers is dense. Simulated dilation of single arterioles is shown to produce both localized and lamina-specific changes in blood flow, while dilation of capillaries in a given retinal vascular layer is shown to lead to increased total flow in that layer. CONCLUSIONS. Our graphing and modeling data suggest that vascular architecture enables both local and lamina-specific control of blood flow in the inner retina. The imaging, graph analysis, and modeling approach presented here will help provide a detailed characterization of vascular changes in a variety of retinal diseases, both in experimental preclinical models and human subjects.

Original languageEnglish (US)
Pages (from-to)5785-5793
Number of pages9
JournalInvestigative Ophthalmology and Visual Science
Volume56
Issue number10
DOIs
StatePublished - Jan 1 2015

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Retinal Vessels
Optical Coherence Tomography
Angiography
Dilatation
Blood Vessels
Retina
Retinal Diseases
Optic Disk
Arterioles
Theoretical Models

Keywords

  • Image processing
  • Imaging methods
  • Retina

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Mapping the 3D connectivity of the rat inner retinal vascular network using OCT angiography. / Leahy, Conor; Radhakrishnan, Harsha; Weiner, Geoffrey; Goldberg, Jeffrey L.; Srinivasan, Vivek.

In: Investigative Ophthalmology and Visual Science, Vol. 56, No. 10, 01.01.2015, p. 5785-5793.

Research output: Contribution to journalArticle

Leahy, Conor ; Radhakrishnan, Harsha ; Weiner, Geoffrey ; Goldberg, Jeffrey L. ; Srinivasan, Vivek. / Mapping the 3D connectivity of the rat inner retinal vascular network using OCT angiography. In: Investigative Ophthalmology and Visual Science. 2015 ; Vol. 56, No. 10. pp. 5785-5793.
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