Mapping QTLs conferring salt tolerance during germination in tomato by selective genotyping

M. R. Foolad, T. Stoltz, C. Dervinis, R. L. Rodriguez, R. A. Jones

Research output: Contribution to journalArticle

62 Scopus citations

Abstract

This study was conducted to identify genomic regions (quantitative trait loci, QTLs) affecting salt tolerance during germination in tomato. Germination response of an F2 population of a cross between UCT5 (Lycopersicon esculentum, salt-sensitive) and LA716 (L. pennellii, salt-tolerant) was evaluated at a salt-stress level of 175 mM NaCl + 17.5 mM CaCl2 (water potential ca. -950 kPa). Germination was scored visually as radicle protrusion at 6 h intervals for 30 consecutive days. Individuals at both extremes of the response distribution (i.e., salt-tolerant and salt-sensitive individuals) were selected. The selected individuals were genotyped at 84 genetic markers including 16 isozymes and 68 restriction fragment length polymorphisms (RFLPs). Trait-based marker analysis (TBA) which measures changes (differences) in marker allele frequencies in selected lines was used to identify marker-linked QTLs. Eight genomic regions were identified on seven tomato chromosomes bearing genes (QTLs) with significant effects on this trait. The results confirmed our previous suggestion that salt tolerance during germination in tomato is polygenically controlled. The salt-tolerant parent contributed favorable QTL alleles on chromosomes 1,3,9 and 12 whereas the salt sensitive parent contributed favorable QTL alleles on chromosomes 2, 7 and 8. The identification of favorable alleles in both parents suggests the likelihood of recovering transgressive segregants in progeny derived from these parental genotypes. The results can be used for marker-assisted selection and breeding of salt-tolerant tomatoes.

Original languageEnglish (US)
Pages (from-to)269-277
Number of pages9
JournalMolecular Breeding
Volume3
Issue number4
StatePublished - 1997

Keywords

  • Lycopersicon
  • Marker-assisted selection (MAS)
  • Quantitative trait loci (QTLs)
  • Restriction fragment length polymorphism (RFLP)
  • Salt tolerance
  • Seed germination

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Horticulture
  • Plant Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology

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    Foolad, M. R., Stoltz, T., Dervinis, C., Rodriguez, R. L., & Jones, R. A. (1997). Mapping QTLs conferring salt tolerance during germination in tomato by selective genotyping. Molecular Breeding, 3(4), 269-277.