Mapping of the thymidine kinase genes of type 1 and type 2 herpes simplex viruses using intertypic recombinants

I. W. Halliburton, Lawrence S Morse, B. Roizman, K. E. Quinn

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The thymidine kinase induced in lytic infection by each of 36 intertypic recombinants of herpes simplex virus (HSV)type 1 and 2 was identified as type 1 or type 2 by studies on the thermolability of enzyme activity, neutralization with type 1 or type 2 antiserum and agar gel immunodiffusion with type 1 or type 2 thymidine kinase antiserum. Fourteen recombinants induced no thymidine kinase, 12 induced type 1 thymidine kinase and 10 induced type 2 thymidine kinase. Correlation of these results with restriction endonuclease analysis of the DNA of the recombinants with five restriction endonucleases (XbaI, EcoRI, HpaI, HsuI, and BglII)allowed mapping of the type 1 thymidine kinase gene at 0.300 to 0.309 map unit and the type thymidine kinase gene at 0.295 to 0.315 map unit.

Original languageEnglish (US)
Pages (from-to)235-253
Number of pages19
JournalJournal of General Virology
Volume49
Issue number2
StatePublished - 1980
Externally publishedYes

Fingerprint

Human Herpesvirus 2
Thymidine Kinase
DNA Restriction Enzymes
Immune Sera
Deoxyribonuclease EcoRI
Genes
Recombinant DNA
Immunodiffusion
Human Herpesvirus 1
Agar
Gels
Enzymes
Infection
thymidine kinase 1
thymidine kinase 2

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Mapping of the thymidine kinase genes of type 1 and type 2 herpes simplex viruses using intertypic recombinants. / Halliburton, I. W.; Morse, Lawrence S; Roizman, B.; Quinn, K. E.

In: Journal of General Virology, Vol. 49, No. 2, 1980, p. 235-253.

Research output: Contribution to journalArticle

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