Mapping of functional domains within the v-erb A oncogene protein: The remnants of the hormone binding domain play multiple, vital roles in protein action

Philip Boucher, Martin L. Privalsky

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

The v-erb A oncogene represents a virally-transduced variant of a thyroid hormone receptor. Biochemical characterization of a series of mutant v-erh A proteins demonstrates that nuclear localization and DNA binding are both necessary for v-erb A function in the neoplastic cell and are mediated by multiple, overlapping domains within the v-erb A polypeptide. Domains of the v-erb A protein necessary for sequence-specific and sequenceindependent DNA binding are distinguishable from one another, and encompass sequences projecting beyond the zinc-finger motifs themselves. Although unable to bind T3 thyroid hormone, the remnants of the hormone binding domain continue to play unanticipated essential roles in v-erb A protein function.

Original languageEnglish (US)
Pages (from-to)1303-1311
Number of pages9
JournalOncogene
Volume5
Issue number9
StatePublished - Sep 1990

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ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research
  • Genetics

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