Mapping of a human brain voltage-gated calcium channel to human chromosome 12p13-pter

William Sun; John Douglas Mcpherson; Danny Q. Hoang; John J. Wasmuth; Glen A. Evans; Mauricio Montal

doi:10.1016/S0888-7543(05)80135-1

Mapping of a human brain voltage-gated calcium channel to human chromosome 12p13-pter

William Sun, John Douglas Mcpherson, Danny Q. Hoang, John J. Wasmuth, Glen A. Evans, Mauricio Montal

Biochemistry and Molecular Medicine

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

Degenerate DNA oligomers coding for highly conserved regions of the voltage-gated calcium channel were synthesized for the polymerase chain reaction (PCR) using DNA from a human brain cDNA library as template. PCR amplified a 640-bp DNA fragment from the human brain cDNA library. Sequencing revealed that this fragment encodes part of a protein highly homologous to a subtype of the dihydropyridine-sensitive calcium channel cloned from rabbit heart and rat brain. Southern analysis of panels of somatic cell hybrids mapped the 640-bp fragment, CACNL1A1, to human chromosome 12p13-pter.

Original language	English (US)
Pages (from-to)	1092-1094
Number of pages	3
Journal	Genomics
Volume	14
Issue number	4
DOIs	https://doi.org/10.1016/S0888-7543(05)80135-1
State	Published - 1992

ASJC Scopus subject areas

Genetics

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title = "Mapping of a human brain voltage-gated calcium channel to human chromosome 12p13-pter",

abstract = "Degenerate DNA oligomers coding for highly conserved regions of the voltage-gated calcium channel were synthesized for the polymerase chain reaction (PCR) using DNA from a human brain cDNA library as template. PCR amplified a 640-bp DNA fragment from the human brain cDNA library. Sequencing revealed that this fragment encodes part of a protein highly homologous to a subtype of the dihydropyridine-sensitive calcium channel cloned from rabbit heart and rat brain. Southern analysis of panels of somatic cell hybrids mapped the 640-bp fragment, CACNL1A1, to human chromosome 12p13-pter.",

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AU - Hoang, Danny Q.

AU - Wasmuth, John J.

AU - Evans, Glen A.

AU - Montal, Mauricio

PY - 1992

Y1 - 1992

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AB - Degenerate DNA oligomers coding for highly conserved regions of the voltage-gated calcium channel were synthesized for the polymerase chain reaction (PCR) using DNA from a human brain cDNA library as template. PCR amplified a 640-bp DNA fragment from the human brain cDNA library. Sequencing revealed that this fragment encodes part of a protein highly homologous to a subtype of the dihydropyridine-sensitive calcium channel cloned from rabbit heart and rat brain. Southern analysis of panels of somatic cell hybrids mapped the 640-bp fragment, CACNL1A1, to human chromosome 12p13-pter.

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Mapping of a human brain voltage-gated calcium channel to human chromosome 12p13-pter

Abstract

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