Abstract
Degenerate DNA oligomers coding for highly conserved regions of the voltage-gated calcium channel were synthesized for the polymerase chain reaction (PCR) using DNA from a human brain cDNA library as template. PCR amplified a 640-bp DNA fragment from the human brain cDNA library. Sequencing revealed that this fragment encodes part of a protein highly homologous to a subtype of the dihydropyridine-sensitive calcium channel cloned from rabbit heart and rat brain. Southern analysis of panels of somatic cell hybrids mapped the 640-bp fragment, CACNL1A1, to human chromosome 12p13-pter.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1092-1094 |
| Number of pages | 3 |
| Journal | Genomics |
| Volume | 14 |
| Issue number | 4 |
| DOIs | |
| State | Published - 1992 |
ASJC Scopus subject areas
- Genetics
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Cite this
Sun, W., Mcpherson, J. D., Hoang, D. Q., Wasmuth, J. J., Evans, G. A., & Montal, M. (1992). Mapping of a human brain voltage-gated calcium channel to human chromosome 12p13-pter. Genomics, 14(4), 1092-1094. https://doi.org/10.1016/S0888-7543(05)80135-1