Abstract
Degenerate DNA oligomers coding for highly conserved regions of the voltage-gated calcium channel were synthesized for the polymerase chain reaction (PCR) using DNA from a human brain cDNA library as template. PCR amplified a 640-bp DNA fragment from the human brain cDNA library. Sequencing revealed that this fragment encodes part of a protein highly homologous to a subtype of the dihydropyridine-sensitive calcium channel cloned from rabbit heart and rat brain. Southern analysis of panels of somatic cell hybrids mapped the 640-bp fragment, CACNL1A1, to human chromosome 12p13-pter.
Original language | English (US) |
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Pages (from-to) | 1092-1094 |
Number of pages | 3 |
Journal | Genomics |
Volume | 14 |
Issue number | 4 |
DOIs | |
State | Published - 1992 |
ASJC Scopus subject areas
- Genetics