Manual hematoxylin and eosin staining of mouse tissue sections

Robert D. Cardiff, Claramae H. Miller, Robert J. Munn

Research output: Contribution to journalArticlepeer-review

321 Scopus citations


The hematoxylin and eosin (H&E) stain is the standard used for microscopic examination of tissues that have been fixed, processed, embedded, and sectioned. It can be performed manually or by automation. For economic reasons, the manual technique is generally the method of choice for facilities with a low sample volume. This protocol describes manual H&E staining of fixed, processed, paraffinembedded, and sectioned mouse tissues. In H&E-stained tissues, the nucleic acids stain dark blue and the proteins stain red to pink or orange. For accurate phenotyping and delineation of tissue detail, the protocol must be adhered to rigorously. This includes frequent reagent changes as well as the use of “in-date” reagents. Appropriate color in a good H&E stain allows for identification of many tissue subtleties that are necessary for accurate diagnosis.

Original languageEnglish (US)
Pages (from-to)655-658
Number of pages4
JournalCold Spring Harbor Protocols
Issue number6
StatePublished - Jan 1 2014
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)


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