Manipulations of microRNA in human pluripotent stem cells and their derivatives

Stephanie N. Rushing, Anthony W. Herren, Deborah Lieu, Ronald A. Li

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

Human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) reprogrammed from somatic cells can self-renew while maintaining their pluripotency to differentiate into virtually all cell types. In addition to their potential for regenerative medicine, hESCs and iPSCs can also serve as excellent in vitro models for the study of human organogenesis and disease models, as well as drug toxicity screening. MicroRNAs (miRNAs) are nonencoding RNAs of ∼22 nucleotides that function as negative transcriptional regulators via degradation or inhibition by RNA interference (RNAi). MiRNAs play essential roles in developmental pathways. This chapter provides a description of how miRNAs can be introduced into hESCs/iPSCs or their derivatives for experiments via lentivirus-mediated gene transfer.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
Pages107-120
Number of pages14
Volume690
DOIs
StatePublished - 2011

Publication series

NameMethods in Molecular Biology
Volume690
ISSN (Print)10643745

Keywords

  • Directed differentiation
  • Human embryonic stem cells
  • MicroRNA
  • RNA interference

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Medicine(all)

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  • Cite this

    Rushing, S. N., Herren, A. W., Lieu, D., & Li, R. A. (2011). Manipulations of microRNA in human pluripotent stem cells and their derivatives. In Methods in Molecular Biology (Vol. 690, pp. 107-120). (Methods in Molecular Biology; Vol. 690). https://doi.org/10.1007/978-1-60761-962-8_7