Manganese binding proteins in human and cow's milk

B. Lonnerdal, Carl L Keen, L. S. Hurley

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

Manganese nutrition in the neonatal period is poorly understood, due in part to a lack of information on the amount of manganese in infant foods and its bioavailability. Since the molecular localization of an element in foods is one determinant of its subsequent bioavailability, we have studied the binding of manganese in human and cow's milk. An extrinsic label of 54Mn was shown to equilibrate isotopically with native manganese in milks and formulas. Milk samples were separated into fat, casein and whey by ultracentrifugation. In human milk, the major part (71%) of manganese was found in whey, 11% in casein and 18% in the lipid fraction. In contrast, in cow's milk, 32% of total manganese was in whey, 67% in casein and 1% in lipid. Within the human whey fraction, most of the manganese was bound to lactoferrin, while in cow's whey, manganese was mostly complexed to ligands with molecular weights less than 200. The distribution of manganese in formulas was closer to that of human milk than of cow's milk. The bioavailability of manganese associated with lactoferrin, casein and low molecular weight complexes needs to be assessed.

Original languageEnglish (US)
Pages (from-to)550-559
Number of pages10
JournalAmerican Journal of Clinical Nutrition
Volume41
Issue number3
StatePublished - 1985

Fingerprint

Human Milk
breast milk
Manganese
manganese
binding proteins
Carrier Proteins
milk
whey
Caseins
casein
Milk
Biological Availability
bioavailability
Lactoferrin
lactoferrin
lipids
Molecular Weight
Infant Food
molecular weight
Lipids

ASJC Scopus subject areas

  • Food Science
  • Medicine (miscellaneous)

Cite this

Lonnerdal, B., Keen, C. L., & Hurley, L. S. (1985). Manganese binding proteins in human and cow's milk. American Journal of Clinical Nutrition, 41(3), 550-559.

Manganese binding proteins in human and cow's milk. / Lonnerdal, B.; Keen, Carl L; Hurley, L. S.

In: American Journal of Clinical Nutrition, Vol. 41, No. 3, 1985, p. 550-559.

Research output: Contribution to journalArticle

Lonnerdal, B, Keen, CL & Hurley, LS 1985, 'Manganese binding proteins in human and cow's milk', American Journal of Clinical Nutrition, vol. 41, no. 3, pp. 550-559.
Lonnerdal, B. ; Keen, Carl L ; Hurley, L. S. / Manganese binding proteins in human and cow's milk. In: American Journal of Clinical Nutrition. 1985 ; Vol. 41, No. 3. pp. 550-559.
@article{1b8c8697e1b24935a1ef6d5d9f9a860c,
title = "Manganese binding proteins in human and cow's milk",
abstract = "Manganese nutrition in the neonatal period is poorly understood, due in part to a lack of information on the amount of manganese in infant foods and its bioavailability. Since the molecular localization of an element in foods is one determinant of its subsequent bioavailability, we have studied the binding of manganese in human and cow's milk. An extrinsic label of 54Mn was shown to equilibrate isotopically with native manganese in milks and formulas. Milk samples were separated into fat, casein and whey by ultracentrifugation. In human milk, the major part (71{\%}) of manganese was found in whey, 11{\%} in casein and 18{\%} in the lipid fraction. In contrast, in cow's milk, 32{\%} of total manganese was in whey, 67{\%} in casein and 1{\%} in lipid. Within the human whey fraction, most of the manganese was bound to lactoferrin, while in cow's whey, manganese was mostly complexed to ligands with molecular weights less than 200. The distribution of manganese in formulas was closer to that of human milk than of cow's milk. The bioavailability of manganese associated with lactoferrin, casein and low molecular weight complexes needs to be assessed.",
author = "B. Lonnerdal and Keen, {Carl L} and Hurley, {L. S.}",
year = "1985",
language = "English (US)",
volume = "41",
pages = "550--559",
journal = "American Journal of Clinical Nutrition",
issn = "0002-9165",
publisher = "American Society for Nutrition",
number = "3",

}

TY - JOUR

T1 - Manganese binding proteins in human and cow's milk

AU - Lonnerdal, B.

AU - Keen, Carl L

AU - Hurley, L. S.

PY - 1985

Y1 - 1985

N2 - Manganese nutrition in the neonatal period is poorly understood, due in part to a lack of information on the amount of manganese in infant foods and its bioavailability. Since the molecular localization of an element in foods is one determinant of its subsequent bioavailability, we have studied the binding of manganese in human and cow's milk. An extrinsic label of 54Mn was shown to equilibrate isotopically with native manganese in milks and formulas. Milk samples were separated into fat, casein and whey by ultracentrifugation. In human milk, the major part (71%) of manganese was found in whey, 11% in casein and 18% in the lipid fraction. In contrast, in cow's milk, 32% of total manganese was in whey, 67% in casein and 1% in lipid. Within the human whey fraction, most of the manganese was bound to lactoferrin, while in cow's whey, manganese was mostly complexed to ligands with molecular weights less than 200. The distribution of manganese in formulas was closer to that of human milk than of cow's milk. The bioavailability of manganese associated with lactoferrin, casein and low molecular weight complexes needs to be assessed.

AB - Manganese nutrition in the neonatal period is poorly understood, due in part to a lack of information on the amount of manganese in infant foods and its bioavailability. Since the molecular localization of an element in foods is one determinant of its subsequent bioavailability, we have studied the binding of manganese in human and cow's milk. An extrinsic label of 54Mn was shown to equilibrate isotopically with native manganese in milks and formulas. Milk samples were separated into fat, casein and whey by ultracentrifugation. In human milk, the major part (71%) of manganese was found in whey, 11% in casein and 18% in the lipid fraction. In contrast, in cow's milk, 32% of total manganese was in whey, 67% in casein and 1% in lipid. Within the human whey fraction, most of the manganese was bound to lactoferrin, while in cow's whey, manganese was mostly complexed to ligands with molecular weights less than 200. The distribution of manganese in formulas was closer to that of human milk than of cow's milk. The bioavailability of manganese associated with lactoferrin, casein and low molecular weight complexes needs to be assessed.

UR - http://www.scopus.com/inward/record.url?scp=0021915014&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021915014&partnerID=8YFLogxK

M3 - Article

C2 - 3872058

AN - SCOPUS:0021915014

VL - 41

SP - 550

EP - 559

JO - American Journal of Clinical Nutrition

JF - American Journal of Clinical Nutrition

SN - 0002-9165

IS - 3

ER -