Manganese and epilepsy: Brain glutamine synthetase and liver arginase activities in genetically epilepsy prone and chronically seizured rats

G. F. Carl, L. K. Blackwell, F. C. Barnett, L. A. Thompson, C. J. Rissinger, K. L. Olin, J. W. Critchfield, Carl L Keen, B. B. Gallagher

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Low blood manganese (Mn2+) concentration is associated with epilepsy in humans and rats. The low Mn2+ concentration is attributed by some investigators to the seizure activity associated with the epilepsy, whereas others propose that the low Mn2+ concentration may be secondary to genetic mechanisms underlying the epilepsy. To begin to differentiate between these possibilities, Mn2+-binding enzymes of liver and brain (i.e., arginase and glutamine synthetase, respectively) were assayed in rats exposed to chronically induced seizures and in genetically epilepsy-prone rats (GEPRs). Chronic seizures caused a decrease in whole blood Mn2+ levels but did not affect brain Mn2+ concentrations. Arginase activity was increased in livers of rats with chronic seizure as compared with controls, but this difference was eliminated when Mn2+ was added to the assay. Brain glutamine synthetase activity was unaffected by chronic seizures, but the activity of this enzyme was significantly lower in GEPR brain than in control brain. Liver arginase activity tended to be lower in GEPRs, although the difference was not statistically significant. These data indicate that seizures affect liver arginase activity through changes in liver Mn2+ concentration, but GEPRs show abnormalities in Mn2+-dependent enzymes apparently independent of seizure activity.

Original languageEnglish (US)
Pages (from-to)441-446
Number of pages6
JournalEpilepsia
Volume34
Issue number3
StatePublished - 1993

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Arginase
Glutamate-Ammonia Ligase
Manganese
Epilepsy
Seizures
Liver
Brain
Enzymes
Research Personnel

Keywords

  • Arginase
  • Convulsions
  • Epilepsy
  • Genetically epilepsy- prone rat
  • Glutamine synthetase
  • Manganese

ASJC Scopus subject areas

  • Clinical Neurology
  • Neuroscience(all)

Cite this

Carl, G. F., Blackwell, L. K., Barnett, F. C., Thompson, L. A., Rissinger, C. J., Olin, K. L., ... Gallagher, B. B. (1993). Manganese and epilepsy: Brain glutamine synthetase and liver arginase activities in genetically epilepsy prone and chronically seizured rats. Epilepsia, 34(3), 441-446.

Manganese and epilepsy : Brain glutamine synthetase and liver arginase activities in genetically epilepsy prone and chronically seizured rats. / Carl, G. F.; Blackwell, L. K.; Barnett, F. C.; Thompson, L. A.; Rissinger, C. J.; Olin, K. L.; Critchfield, J. W.; Keen, Carl L; Gallagher, B. B.

In: Epilepsia, Vol. 34, No. 3, 1993, p. 441-446.

Research output: Contribution to journalArticle

Carl, GF, Blackwell, LK, Barnett, FC, Thompson, LA, Rissinger, CJ, Olin, KL, Critchfield, JW, Keen, CL & Gallagher, BB 1993, 'Manganese and epilepsy: Brain glutamine synthetase and liver arginase activities in genetically epilepsy prone and chronically seizured rats', Epilepsia, vol. 34, no. 3, pp. 441-446.
Carl GF, Blackwell LK, Barnett FC, Thompson LA, Rissinger CJ, Olin KL et al. Manganese and epilepsy: Brain glutamine synthetase and liver arginase activities in genetically epilepsy prone and chronically seizured rats. Epilepsia. 1993;34(3):441-446.
Carl, G. F. ; Blackwell, L. K. ; Barnett, F. C. ; Thompson, L. A. ; Rissinger, C. J. ; Olin, K. L. ; Critchfield, J. W. ; Keen, Carl L ; Gallagher, B. B. / Manganese and epilepsy : Brain glutamine synthetase and liver arginase activities in genetically epilepsy prone and chronically seizured rats. In: Epilepsia. 1993 ; Vol. 34, No. 3. pp. 441-446.
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