Mammalian proteasome subpopulations with distinct molecular compositions and proteolytic activities

Oliver Drews, Robert Wildgruber, Chenggong Zong, Ute Sukop, Mikkel Nissum, Gerhard Weber, Aldrin V Gomes, Peipei Ping

Research output: Contribution to journalArticle

95 Scopus citations

Abstract

The proteasome-dependent protein degradation participates in multiple essential cellular processes. Modulation of proteasomal activities may alter cardiac function and disease phenotypes. However, cardiovascular studies reported thus far have yielded conflicting results. We hypothesized that a contributing factor to the contradicting literature may be caused by existing proteasome heterogeneity in the myocardium. In this investigation, we provide the very first direct demonstration of distinct proteasome subpopulations in murine hearts. The cardiac proteasome subpopulations differ in their molecular compositions and proteolytic activities. Furthermore they were distinguished from proteasome subpopulations identified in murine livers. The study was facilitated by the development of novel protocols for in-solution isoelectric focusing of multiprotein complexes in a laminar flow that support an average resolution of 0.04 pH units. Utilizing these protocols, the majority of cardiac proteasome complexes displayed an isoelectric point of 5.26 with additional subpopulations focusing in the range from pH 5.10 to 5.33. In contrast, the majority of hepatic 20 S proteasomes had a pl of 5.05 and focused from pH 5.01 to 5.29. Importantly proteasome subpopulations degraded specific model peptides with different turnover rates. Among cardiac subpopulations, proteasomes with an approximate pl of 5.21 showed 40% higher trypsin-like activity than those with pl 5.28. Distinct proteasome assembly may be a contributing factor to variations in proteolytic activities because proteasomes with pl 5.21 contained 58% less of the inducible subunit β2i compared with those with pl 5.28. In addition, dephosphorylation of 20 S proteasomes demonstrated that besides molecular composition posttranslational modifications largely contribute to their pl values. These data suggest the possibility of mixed 20 S proteasome assembly, a departure from the currently hypothesized two subpopulations: constitutive and immuno forms. The identification of multiple distinct proteasome subpopulations in heart provides key mechanistic insights for achieving selective and targeted regulation of this essential protein degradation machinery. Thus, proteasome subpopulations may serve as novel therapeutic targets in the myocardium.

Original languageEnglish (US)
Pages (from-to)2021-2031
Number of pages11
JournalMolecular and Cellular Proteomics
Volume6
Issue number11
DOIs
StatePublished - Nov 2007

ASJC Scopus subject areas

  • Biochemistry

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    Drews, O., Wildgruber, R., Zong, C., Sukop, U., Nissum, M., Weber, G., Gomes, A. V., & Ping, P. (2007). Mammalian proteasome subpopulations with distinct molecular compositions and proteolytic activities. Molecular and Cellular Proteomics, 6(11), 2021-2031. https://doi.org/10.1074/mcp.M700187-MCP200