Male-to-male differences in post-thaw motility of rhesus spermatozoa after cryopreservation of replicate ejaculates.

S. P. Leibo, H. Michael Kubisch, R. Dee Schramm, Richard M. Harrison, Catherine A. VandeVoort

Research output: Contribution to journalArticle

28 Scopus citations

Abstract

BACKGROUND: The efficiency of controlled propagation to produce rhesus monkeys of particular genotypes can be maximized by use of cryopreserved spermatozoa collected from specific males to inseminate appropriate females. But this assumes that semen from males with different genotypes can be cryopreserved with equal effectiveness. METHODS: To investigate whether spermatozoa from different Macaca mulatta males can be effectively cryopreserved when frozen under identical conditions, we collected and froze semen specimens from 13 adult, fertile males maintained at three primate research centers. RESULTS: Survival, based on post-thaw motility normalized to the pre-freeze value, was assayed within 30 minutes after thawing; it varied from 50% to 70% but declined thereafter. To examine the response of semen from individual males, we collected and froze three to six ejaculates per male from each of seven males. CONCLUSIONS: In general, semen from a given male responded reproducibly to freezing, but there were significant differences among males. The cause of these differences among M. mulatta males in post-thaw sperm survival remains unidentified.

Original languageEnglish (US)
Pages (from-to)151-163
Number of pages13
JournalJournal of Medical Primatology
Volume36
Issue number3
DOIs
StatePublished - Jun 2007
Externally publishedYes

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

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