Male infertility, impaired spermatogenesis, and azoospermia in mice deficient for the pseudophosphatase Sbf1

Ron Firestein, Peter L. Nagy, Megan E Daly, Phil Huie, Marco Conti, Michael L. Cleary

Research output: Contribution to journalArticle

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Abstract

Pseudophosphatases display extensive sequence similarities to phosphatases but harbor amino acid alterations in their active-site consensus motifs that render them catalytically inactive. A potential role in substrate trapping or docking has been proposed, but the specific requirements for pseudophosphatases during development and differentiation are unknown. We demonstrate here that Sbf1, a pseudophosphatase of the myotubularin family, is expressed at high levels in seminiferous tubules of the testis, specifically in Sertoli's cells, spermatogonia, and pachytene spermatocytes, but not in post-meiotic round spermatids. Mice that are nullizygous for Sbf1 exhibit male infertility characterized by azoospermia. The onset of the spermatogenic defect occurs in the first wave of spermatogenesis at 17 days after birth during the synchronized progression of pachytene spermatocytes to haploid spermatids. Vacuolation of the Sertoli's cells is the earliest observed phenotype and is followed by reduced formation of spermatids and eventual depletion of the germ cell compartment in older mice. The nullizygous phenotype in conjunction with high-level expression of Sbf1 in premeiotic germ cells and Sertoli's cells is consistent with a crucial role for Sbf1 in transition from diploid to haploid spermatocytes. These studies demonstrate an essential role for a pseudophosphatase and implicate signaling pathways regulated by myotubularin family proteins in spermatogenesis and germ cell differentiation.

Original languageEnglish (US)
Pages (from-to)1165-1172
Number of pages8
JournalJournal of Clinical Investigation
Volume109
Issue number9
DOIs
StatePublished - 2002
Externally publishedYes

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Azoospermia
Spermatocytes
Spermatids
Sertoli Cells
Male Infertility
Spermatogenesis
Germ Cells
Haploidy
Phenotype
Spermatogonia
Seminiferous Tubules
Diploidy
Phosphoric Monoester Hydrolases
Testis
Cell Differentiation
Catalytic Domain
Parturition
Amino Acids
Proteins
myotubularin

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Male infertility, impaired spermatogenesis, and azoospermia in mice deficient for the pseudophosphatase Sbf1. / Firestein, Ron; Nagy, Peter L.; Daly, Megan E; Huie, Phil; Conti, Marco; Cleary, Michael L.

In: Journal of Clinical Investigation, Vol. 109, No. 9, 2002, p. 1165-1172.

Research output: Contribution to journalArticle

Firestein, Ron ; Nagy, Peter L. ; Daly, Megan E ; Huie, Phil ; Conti, Marco ; Cleary, Michael L. / Male infertility, impaired spermatogenesis, and azoospermia in mice deficient for the pseudophosphatase Sbf1. In: Journal of Clinical Investigation. 2002 ; Vol. 109, No. 9. pp. 1165-1172.
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