Making and using calcium-selective mini- and microelectrodes

L. Hove-Madsen, S. Baudet, Donald M Bers

Research output: Book/ReportBook

10 Citations (Scopus)

Abstract

Detection and measurement of intracellular calcium concentration ([Ca2+]i) have relied on various methods, the popularity of which depends on their ease of use and applicability to different cell types. Historically, Ca2+-selective electrodes have been used concomitantly with absorption indicators such as arsenazo-III, but their interest has been eclipsed by the introduction of a large number of fluorescent calcium probes with calcium sensitivities varying from the nanomolar to the micromolar range such as fura-2, indo-1, fluo-4, and many others. In this chapter, we emphasize the utility of Ca2+-selective electrodes and show that their use is complementary to use of fluorescent indicators; indeed, each method has advantages and disadvantages. We first describe the preparation and application of Ca2+-selective minielectrodes based on the Ca2+ ligand ETH 129 (Schefer et al., 1986) that have a larger dynamic range and faster response time than most commercially available calcium electrodes. The second part of the chapter is dedicated to ETH 129-based Ca2+-selective microelectrodes (MEs), and their application in the determination of [Ca2+]i in cardiac cells. Since numerous reviews and books have been dedicated to the theoretical aspects of ion-selective ME principles and technology, this chapter is not intended for investigators who have no experience with MEs.

Original languageEnglish (US)
PublisherUnknown Publisher
Number of pages23
Volume99
EditionC
DOIs
StatePublished - 2010

Publication series

NameMethods in Cell Biology
No.C
Volume99
ISSN (Print)0091679X

Fingerprint

Microelectrodes
Calcium
Electrodes
Arsenazo III
Fura-2
Fluorescent Dyes
Reaction Time
Research Personnel
Ions
Ligands
Technology
N,N,N',N'-tetracyclohexyl-3-oxapentanediamide

ASJC Scopus subject areas

  • Cell Biology

Cite this

Hove-Madsen, L., Baudet, S., & Bers, D. M. (2010). Making and using calcium-selective mini- and microelectrodes. (C ed.) (Methods in Cell Biology; Vol. 99, No. C). Unknown Publisher. https://doi.org/10.1016/B978-0-12-374841-6.00003-7

Making and using calcium-selective mini- and microelectrodes. / Hove-Madsen, L.; Baudet, S.; Bers, Donald M.

C ed. Unknown Publisher, 2010. 23 p. (Methods in Cell Biology; Vol. 99, No. C).

Research output: Book/ReportBook

Hove-Madsen, L, Baudet, S & Bers, DM 2010, Making and using calcium-selective mini- and microelectrodes. Methods in Cell Biology, no. C, vol. 99, vol. 99, C edn, Unknown Publisher. https://doi.org/10.1016/B978-0-12-374841-6.00003-7
Hove-Madsen L, Baudet S, Bers DM. Making and using calcium-selective mini- and microelectrodes. C ed. Unknown Publisher, 2010. 23 p. (Methods in Cell Biology; C). https://doi.org/10.1016/B978-0-12-374841-6.00003-7
Hove-Madsen, L. ; Baudet, S. ; Bers, Donald M. / Making and using calcium-selective mini- and microelectrodes. C ed. Unknown Publisher, 2010. 23 p. (Methods in Cell Biology; C).
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