Making and using calcium-selective mini- and microelectrodes

L. Hove-Madsen, S. Baudet, Donald M Bers

Research output: Book/ReportBook

13 Scopus citations


Detection and measurement of intracellular calcium concentration ([Ca2+]i) have relied on various methods, the popularity of which depends on their ease of use and applicability to different cell types. Historically, Ca2+-selective electrodes have been used concomitantly with absorption indicators such as arsenazo-III, but their interest has been eclipsed by the introduction of a large number of fluorescent calcium probes with calcium sensitivities varying from the nanomolar to the micromolar range such as fura-2, indo-1, fluo-4, and many others. In this chapter, we emphasize the utility of Ca2+-selective electrodes and show that their use is complementary to use of fluorescent indicators; indeed, each method has advantages and disadvantages. We first describe the preparation and application of Ca2+-selective minielectrodes based on the Ca2+ ligand ETH 129 (Schefer et al., 1986) that have a larger dynamic range and faster response time than most commercially available calcium electrodes. The second part of the chapter is dedicated to ETH 129-based Ca2+-selective microelectrodes (MEs), and their application in the determination of [Ca2+]i in cardiac cells. Since numerous reviews and books have been dedicated to the theoretical aspects of ion-selective ME principles and technology, this chapter is not intended for investigators who have no experience with MEs.

Original languageEnglish (US)
PublisherUnknown Publisher
Number of pages23
StatePublished - 2010

Publication series

NameMethods in Cell Biology
ISSN (Print)0091679X

ASJC Scopus subject areas

  • Cell Biology


Dive into the research topics of 'Making and using calcium-selective mini- and microelectrodes'. Together they form a unique fingerprint.

Cite this