Maintaince of the relative proportion of oligodendrocytes to axons even in the absence of BAX and BAK

Kumi Kawai, Takayuki Ito, Aki Itoh, Makoto Horiuchi, Kouji Wakayama, Peter Bannerman, James Y. Garbern, David E Pleasure, Tullia Lindsten

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Highly purified oligodendroglial lineage cells from mice lacking functional bax and bak genes were resistant to apoptosis after in-vitro differentiation, indicating an essential role of the intrinsic apoptotic pathway in apoptosis of oligodendrocytes in the absence of neurons (axons) and other glial cells. These mice therefore provide a valuable tool with which to evaluate the significance of the intrinsic apoptotic pathway in regulating the population sizes of oligodendrocytes and oligodendroglial progenitor cells. Quantitative analysis of the optic nerves and the dorsal columns of the spinal cord revealed that the absolute numbers of mature oligodendrocytes immunolabeled for aspartoacylase and adult glial progenitor cells expressing NG2 chondroitin sulfate proteoglycan were increased in both white matter tracts of adult bax/bak-deficient mice and, to a lesser extent, bax-deficient mice, except that there was no increase in NG2-positive progenitor cells in the dorsal columns of these strains of mutant mice. These increases in mature oligodendrocytes and progenitor cells in bax/bak-deficient mice were unexpectedly proportional to increases in numbers of axons in these white matter tracts, thus retaining the oligodendroglial lineage to axon ratios of at most 1.3-fold of the physiological numbers. This is in contrast to the prominent expansion in numbers of neural precursor cells in the subventricular zones of these adult mutant mice. Our study indicates that homeostatic control of cell number is different for progenitors of the oligodendroglial and neuronal lineages. Furthermore, regulatory mechanism(s) operating in addition to apoptotic elimination through the intrinsic pathway, appear to prevent the overproduction of highly mitotic oligodendroglial progenitor cells.

Original languageEnglish (US)
Pages (from-to)2030-2041
Number of pages12
JournalEuropean Journal of Neuroscience
Volume30
Issue number11
DOIs
StatePublished - Dec 2009

Fingerprint

Oligodendroglia
Axons
Stem Cells
Neuroglia
Mutant Strains Mice
Apoptosis
Chondroitin Sulfate Proteoglycans
Lateral Ventricles
Optic Nerve
Population Density
Spinal Cord
Cell Count
Neurons
Genes

Keywords

  • Apoptosis
  • Aspartoacylase
  • Bcl-2-related protein family
  • Differentiation
  • Mouse
  • NG2

ASJC Scopus subject areas

  • Neuroscience(all)
  • Medicine(all)

Cite this

Kawai, K., Ito, T., Itoh, A., Horiuchi, M., Wakayama, K., Bannerman, P., ... Lindsten, T. (2009). Maintaince of the relative proportion of oligodendrocytes to axons even in the absence of BAX and BAK. European Journal of Neuroscience, 30(11), 2030-2041. https://doi.org/10.1111/j.1460-9568.2009.06988.x

Maintaince of the relative proportion of oligodendrocytes to axons even in the absence of BAX and BAK. / Kawai, Kumi; Ito, Takayuki; Itoh, Aki; Horiuchi, Makoto; Wakayama, Kouji; Bannerman, Peter; Garbern, James Y.; Pleasure, David E; Lindsten, Tullia.

In: European Journal of Neuroscience, Vol. 30, No. 11, 12.2009, p. 2030-2041.

Research output: Contribution to journalArticle

Kawai, K, Ito, T, Itoh, A, Horiuchi, M, Wakayama, K, Bannerman, P, Garbern, JY, Pleasure, DE & Lindsten, T 2009, 'Maintaince of the relative proportion of oligodendrocytes to axons even in the absence of BAX and BAK', European Journal of Neuroscience, vol. 30, no. 11, pp. 2030-2041. https://doi.org/10.1111/j.1460-9568.2009.06988.x
Kawai, Kumi ; Ito, Takayuki ; Itoh, Aki ; Horiuchi, Makoto ; Wakayama, Kouji ; Bannerman, Peter ; Garbern, James Y. ; Pleasure, David E ; Lindsten, Tullia. / Maintaince of the relative proportion of oligodendrocytes to axons even in the absence of BAX and BAK. In: European Journal of Neuroscience. 2009 ; Vol. 30, No. 11. pp. 2030-2041.
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