MAGNETIC FIELD FOR SEPARATION AND ANALYSIS OF LIVING CELLS.

M. Zborowski, P. S. Malchesky, S. R. Savon, Ralph Green, G. S. Hall, J. J. Lewandowski, Y. Nose

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

The novel slide ferrography scheme, combined with the use of the strongest permanent magnets available and capillary flow of the sample, showed that the chemistry of trivalent erbium and ferritin reaction with the cell membrane allows for the paramagnetic separation of a wide variety of cells (human, animal, bacteria) in different media. Results indicated that there is a differential binding of trivalent erbium to native and cultured human mononuclear blood cells. Labeling with cationized ferritin caused the magnetic deposition of all cells studied, i. e. native and cultured human lymphocytes, and mouse lymphoma cells (YAC-1). Modified slide ferrography shows that the bacterium E. coli has a high affinity for erbium ions and becomes readily magnetized and separated. Scattered light intensity-correlated to the bacterial cell concentration in the samples was analyzed by ferrography.

Original languageEnglish (US)
Title of host publicationIEEE/Engineering in Medicine and Biology Society Annual Conference
Place of PublicationNew York, NY, USA
PublisherIEEE
Pages1161-1162
Number of pages2
StatePublished - 1987
Externally publishedYes

Fingerprint

Erbium
Cells
Magnetic fields
Bacteria
Capillary flow
Lymphocytes
Cell membranes
Labeling
Escherichia coli
Permanent magnets
Animals
Blood
Ions

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Zborowski, M., Malchesky, P. S., Savon, S. R., Green, R., Hall, G. S., Lewandowski, J. J., & Nose, Y. (1987). MAGNETIC FIELD FOR SEPARATION AND ANALYSIS OF LIVING CELLS. In IEEE/Engineering in Medicine and Biology Society Annual Conference (pp. 1161-1162). New York, NY, USA: IEEE.

MAGNETIC FIELD FOR SEPARATION AND ANALYSIS OF LIVING CELLS. / Zborowski, M.; Malchesky, P. S.; Savon, S. R.; Green, Ralph; Hall, G. S.; Lewandowski, J. J.; Nose, Y.

IEEE/Engineering in Medicine and Biology Society Annual Conference. New York, NY, USA : IEEE, 1987. p. 1161-1162.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Zborowski, M, Malchesky, PS, Savon, SR, Green, R, Hall, GS, Lewandowski, JJ & Nose, Y 1987, MAGNETIC FIELD FOR SEPARATION AND ANALYSIS OF LIVING CELLS. in IEEE/Engineering in Medicine and Biology Society Annual Conference. IEEE, New York, NY, USA, pp. 1161-1162.
Zborowski M, Malchesky PS, Savon SR, Green R, Hall GS, Lewandowski JJ et al. MAGNETIC FIELD FOR SEPARATION AND ANALYSIS OF LIVING CELLS. In IEEE/Engineering in Medicine and Biology Society Annual Conference. New York, NY, USA: IEEE. 1987. p. 1161-1162
Zborowski, M. ; Malchesky, P. S. ; Savon, S. R. ; Green, Ralph ; Hall, G. S. ; Lewandowski, J. J. ; Nose, Y. / MAGNETIC FIELD FOR SEPARATION AND ANALYSIS OF LIVING CELLS. IEEE/Engineering in Medicine and Biology Society Annual Conference. New York, NY, USA : IEEE, 1987. pp. 1161-1162
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abstract = "The novel slide ferrography scheme, combined with the use of the strongest permanent magnets available and capillary flow of the sample, showed that the chemistry of trivalent erbium and ferritin reaction with the cell membrane allows for the paramagnetic separation of a wide variety of cells (human, animal, bacteria) in different media. Results indicated that there is a differential binding of trivalent erbium to native and cultured human mononuclear blood cells. Labeling with cationized ferritin caused the magnetic deposition of all cells studied, i. e. native and cultured human lymphocytes, and mouse lymphoma cells (YAC-1). Modified slide ferrography shows that the bacterium E. coli has a high affinity for erbium ions and becomes readily magnetized and separated. Scattered light intensity-correlated to the bacterial cell concentration in the samples was analyzed by ferrography.",
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