MAGNETIC FIELD FOR SEPARATION AND ANALYSIS OF LIVING CELLS.

M. Zborowski, P. S. Malchesky, S. R. Savon, Ralph Green, G. S. Hall, J. J. Lewandowski, Y. Nose

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Scopus citations

Abstract

The novel slide ferrography scheme, combined with the use of the strongest permanent magnets available and capillary flow of the sample, showed that the chemistry of trivalent erbium and ferritin reaction with the cell membrane allows for the paramagnetic separation of a wide variety of cells (human, animal, bacteria) in different media. Results indicated that there is a differential binding of trivalent erbium to native and cultured human mononuclear blood cells. Labeling with cationized ferritin caused the magnetic deposition of all cells studied, i. e. native and cultured human lymphocytes, and mouse lymphoma cells (YAC-1). Modified slide ferrography shows that the bacterium E. coli has a high affinity for erbium ions and becomes readily magnetized and separated. Scattered light intensity-correlated to the bacterial cell concentration in the samples was analyzed by ferrography.

Original languageEnglish (US)
Title of host publicationIEEE/Engineering in Medicine and Biology Society Annual Conference
Place of PublicationNew York, NY, USA
PublisherIEEE
Pages1161-1162
Number of pages2
StatePublished - 1987
Externally publishedYes

ASJC Scopus subject areas

  • Engineering(all)

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