MAGI-2 in prostate cancer: An immunohistochemical study

Jeffery Goldstein, Alexander D Borowsky, Rajen Goyal, Joseph T. Roland, Shanna A. Arnold, Lan L. Gellert, Peter E. Clark, Omar Hameed, Giovanna A. Giannico

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2 (MAGI-2) is a scaffolding protein that links cell adhesion molecules, receptors, and signaling molecules to the cytoskeleton and maintains the architecture of cell junctions. MAGI-2 gene rearrangements have recently been described in prostate cancer. We studied the immunohistochemical expression of MAGI-2 protein in prostate tissue. Seventy-eight radical prostatectomies were used to construct 3 tissue microarrays consisting of 512 cores, including benign tissue, benign prostatic hyperplasia, high-grade prostatic intraepithelial neoplasia (HGPIN), and adenocarcinoma, Gleason patterns 3 to 5. Immunohistochemistry for phosphatase and tensin homologue (PTEN) and double-stain MAGI-2/p63 was performed and analyzed by visual and image analysis, the latter as percent of analyzed area (%AREA), and mean optical density multiplied by %AREA (STAIN). By visual and image analysis, MAGI-2 was significantly higher in adenocarcinoma and HGPIN compared with benign (benign versus HGPIN P <.001; benign versus adenocarcinoma, P <.001). HGPIN and adenocarcinoma did not significantly differ by either modality. Using visual intensity to distinguish benign tissue and adenocarcinoma, a receiver operating curve yielded an area under the curve of 0.902. A STAIN threshold of 1470 yielded a sensitivity of 0.66 and specificity of 0.96. There was a significant correlation between PTEN and MAGI-2 staining for normal and benign prostatic hyperplasia, but this was lost in HGPIN and cancer. We conclude that MAGI-2 immunoreactivity is elevated in prostate cancer and HGPIN compared with normal tissue, and suggest that MAGI-2 may contribute to prostate carcinogenesis. This is the first report of MAGI-2 staining by immunohistochemistry in prostate cancer.

Original languageEnglish (US)
Pages (from-to)83-91
Number of pages9
JournalHuman Pathology
Volume52
DOIs
StatePublished - Jun 1 2016

Keywords

  • Image analysis
  • Immunohistochemistry
  • MAGI-2
  • Prostate cancer
  • PTEN

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Medicine(all)

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  • Cite this

    Goldstein, J., Borowsky, A. D., Goyal, R., Roland, J. T., Arnold, S. A., Gellert, L. L., Clark, P. E., Hameed, O., & Giannico, G. A. (2016). MAGI-2 in prostate cancer: An immunohistochemical study. Human Pathology, 52, 83-91. https://doi.org/10.1016/j.humpath.2016.01.003