MAGI-2 in prostate cancer

An immunohistochemical study

Jeffery Goldstein, Alexander D Borowsky, Rajen Goyal, Joseph T. Roland, Shanna A. Arnold, Lan L. Gellert, Peter E. Clark, Omar Hameed, Giovanna A. Giannico

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2 (MAGI-2) is a scaffolding protein that links cell adhesion molecules, receptors, and signaling molecules to the cytoskeleton and maintains the architecture of cell junctions. MAGI-2 gene rearrangements have recently been described in prostate cancer. We studied the immunohistochemical expression of MAGI-2 protein in prostate tissue. Seventy-eight radical prostatectomies were used to construct 3 tissue microarrays consisting of 512 cores, including benign tissue, benign prostatic hyperplasia, high-grade prostatic intraepithelial neoplasia (HGPIN), and adenocarcinoma, Gleason patterns 3 to 5. Immunohistochemistry for phosphatase and tensin homologue (PTEN) and double-stain MAGI-2/p63 was performed and analyzed by visual and image analysis, the latter as percent of analyzed area (%AREA), and mean optical density multiplied by %AREA (STAIN). By visual and image analysis, MAGI-2 was significantly higher in adenocarcinoma and HGPIN compared with benign (benign versus HGPIN P <.001; benign versus adenocarcinoma, P <.001). HGPIN and adenocarcinoma did not significantly differ by either modality. Using visual intensity to distinguish benign tissue and adenocarcinoma, a receiver operating curve yielded an area under the curve of 0.902. A STAIN threshold of 1470 yielded a sensitivity of 0.66 and specificity of 0.96. There was a significant correlation between PTEN and MAGI-2 staining for normal and benign prostatic hyperplasia, but this was lost in HGPIN and cancer. We conclude that MAGI-2 immunoreactivity is elevated in prostate cancer and HGPIN compared with normal tissue, and suggest that MAGI-2 may contribute to prostate carcinogenesis. This is the first report of MAGI-2 staining by immunohistochemistry in prostate cancer.

Original languageEnglish (US)
Pages (from-to)83-91
Number of pages9
JournalHuman Pathology
Volume52
DOIs
StatePublished - Jun 1 2016

Fingerprint

Prostatic Intraepithelial Neoplasia
Prostatic Neoplasms
Adenocarcinoma
Prostatic Hyperplasia
Phosphoric Monoester Hydrolases
Prostate
Immunohistochemistry
Guanylate Kinases
Staining and Labeling
PDZ Domains
Intercellular Junctions
Gene Rearrangement
Cell Adhesion Molecules
Prostatectomy
Cytoskeleton
Area Under Curve
Carcinogenesis
Proteins
Coloring Agents
Sensitivity and Specificity

Keywords

  • Image analysis
  • Immunohistochemistry
  • MAGI-2
  • Prostate cancer
  • PTEN

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Medicine(all)

Cite this

Goldstein, J., Borowsky, A. D., Goyal, R., Roland, J. T., Arnold, S. A., Gellert, L. L., ... Giannico, G. A. (2016). MAGI-2 in prostate cancer: An immunohistochemical study. Human Pathology, 52, 83-91. https://doi.org/10.1016/j.humpath.2016.01.003

MAGI-2 in prostate cancer : An immunohistochemical study. / Goldstein, Jeffery; Borowsky, Alexander D; Goyal, Rajen; Roland, Joseph T.; Arnold, Shanna A.; Gellert, Lan L.; Clark, Peter E.; Hameed, Omar; Giannico, Giovanna A.

In: Human Pathology, Vol. 52, 01.06.2016, p. 83-91.

Research output: Contribution to journalArticle

Goldstein, J, Borowsky, AD, Goyal, R, Roland, JT, Arnold, SA, Gellert, LL, Clark, PE, Hameed, O & Giannico, GA 2016, 'MAGI-2 in prostate cancer: An immunohistochemical study', Human Pathology, vol. 52, pp. 83-91. https://doi.org/10.1016/j.humpath.2016.01.003
Goldstein J, Borowsky AD, Goyal R, Roland JT, Arnold SA, Gellert LL et al. MAGI-2 in prostate cancer: An immunohistochemical study. Human Pathology. 2016 Jun 1;52:83-91. https://doi.org/10.1016/j.humpath.2016.01.003
Goldstein, Jeffery ; Borowsky, Alexander D ; Goyal, Rajen ; Roland, Joseph T. ; Arnold, Shanna A. ; Gellert, Lan L. ; Clark, Peter E. ; Hameed, Omar ; Giannico, Giovanna A. / MAGI-2 in prostate cancer : An immunohistochemical study. In: Human Pathology. 2016 ; Vol. 52. pp. 83-91.
@article{e4d386ded7114ccc834a6d3334f9e28c,
title = "MAGI-2 in prostate cancer: An immunohistochemical study",
abstract = "Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2 (MAGI-2) is a scaffolding protein that links cell adhesion molecules, receptors, and signaling molecules to the cytoskeleton and maintains the architecture of cell junctions. MAGI-2 gene rearrangements have recently been described in prostate cancer. We studied the immunohistochemical expression of MAGI-2 protein in prostate tissue. Seventy-eight radical prostatectomies were used to construct 3 tissue microarrays consisting of 512 cores, including benign tissue, benign prostatic hyperplasia, high-grade prostatic intraepithelial neoplasia (HGPIN), and adenocarcinoma, Gleason patterns 3 to 5. Immunohistochemistry for phosphatase and tensin homologue (PTEN) and double-stain MAGI-2/p63 was performed and analyzed by visual and image analysis, the latter as percent of analyzed area ({\%}AREA), and mean optical density multiplied by {\%}AREA (STAIN). By visual and image analysis, MAGI-2 was significantly higher in adenocarcinoma and HGPIN compared with benign (benign versus HGPIN P <.001; benign versus adenocarcinoma, P <.001). HGPIN and adenocarcinoma did not significantly differ by either modality. Using visual intensity to distinguish benign tissue and adenocarcinoma, a receiver operating curve yielded an area under the curve of 0.902. A STAIN threshold of 1470 yielded a sensitivity of 0.66 and specificity of 0.96. There was a significant correlation between PTEN and MAGI-2 staining for normal and benign prostatic hyperplasia, but this was lost in HGPIN and cancer. We conclude that MAGI-2 immunoreactivity is elevated in prostate cancer and HGPIN compared with normal tissue, and suggest that MAGI-2 may contribute to prostate carcinogenesis. This is the first report of MAGI-2 staining by immunohistochemistry in prostate cancer.",
keywords = "Image analysis, Immunohistochemistry, MAGI-2, Prostate cancer, PTEN",
author = "Jeffery Goldstein and Borowsky, {Alexander D} and Rajen Goyal and Roland, {Joseph T.} and Arnold, {Shanna A.} and Gellert, {Lan L.} and Clark, {Peter E.} and Omar Hameed and Giannico, {Giovanna A.}",
year = "2016",
month = "6",
day = "1",
doi = "10.1016/j.humpath.2016.01.003",
language = "English (US)",
volume = "52",
pages = "83--91",
journal = "Human Pathology",
issn = "0046-8177",
publisher = "W.B. Saunders Ltd",

}

TY - JOUR

T1 - MAGI-2 in prostate cancer

T2 - An immunohistochemical study

AU - Goldstein, Jeffery

AU - Borowsky, Alexander D

AU - Goyal, Rajen

AU - Roland, Joseph T.

AU - Arnold, Shanna A.

AU - Gellert, Lan L.

AU - Clark, Peter E.

AU - Hameed, Omar

AU - Giannico, Giovanna A.

PY - 2016/6/1

Y1 - 2016/6/1

N2 - Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2 (MAGI-2) is a scaffolding protein that links cell adhesion molecules, receptors, and signaling molecules to the cytoskeleton and maintains the architecture of cell junctions. MAGI-2 gene rearrangements have recently been described in prostate cancer. We studied the immunohistochemical expression of MAGI-2 protein in prostate tissue. Seventy-eight radical prostatectomies were used to construct 3 tissue microarrays consisting of 512 cores, including benign tissue, benign prostatic hyperplasia, high-grade prostatic intraepithelial neoplasia (HGPIN), and adenocarcinoma, Gleason patterns 3 to 5. Immunohistochemistry for phosphatase and tensin homologue (PTEN) and double-stain MAGI-2/p63 was performed and analyzed by visual and image analysis, the latter as percent of analyzed area (%AREA), and mean optical density multiplied by %AREA (STAIN). By visual and image analysis, MAGI-2 was significantly higher in adenocarcinoma and HGPIN compared with benign (benign versus HGPIN P <.001; benign versus adenocarcinoma, P <.001). HGPIN and adenocarcinoma did not significantly differ by either modality. Using visual intensity to distinguish benign tissue and adenocarcinoma, a receiver operating curve yielded an area under the curve of 0.902. A STAIN threshold of 1470 yielded a sensitivity of 0.66 and specificity of 0.96. There was a significant correlation between PTEN and MAGI-2 staining for normal and benign prostatic hyperplasia, but this was lost in HGPIN and cancer. We conclude that MAGI-2 immunoreactivity is elevated in prostate cancer and HGPIN compared with normal tissue, and suggest that MAGI-2 may contribute to prostate carcinogenesis. This is the first report of MAGI-2 staining by immunohistochemistry in prostate cancer.

AB - Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2 (MAGI-2) is a scaffolding protein that links cell adhesion molecules, receptors, and signaling molecules to the cytoskeleton and maintains the architecture of cell junctions. MAGI-2 gene rearrangements have recently been described in prostate cancer. We studied the immunohistochemical expression of MAGI-2 protein in prostate tissue. Seventy-eight radical prostatectomies were used to construct 3 tissue microarrays consisting of 512 cores, including benign tissue, benign prostatic hyperplasia, high-grade prostatic intraepithelial neoplasia (HGPIN), and adenocarcinoma, Gleason patterns 3 to 5. Immunohistochemistry for phosphatase and tensin homologue (PTEN) and double-stain MAGI-2/p63 was performed and analyzed by visual and image analysis, the latter as percent of analyzed area (%AREA), and mean optical density multiplied by %AREA (STAIN). By visual and image analysis, MAGI-2 was significantly higher in adenocarcinoma and HGPIN compared with benign (benign versus HGPIN P <.001; benign versus adenocarcinoma, P <.001). HGPIN and adenocarcinoma did not significantly differ by either modality. Using visual intensity to distinguish benign tissue and adenocarcinoma, a receiver operating curve yielded an area under the curve of 0.902. A STAIN threshold of 1470 yielded a sensitivity of 0.66 and specificity of 0.96. There was a significant correlation between PTEN and MAGI-2 staining for normal and benign prostatic hyperplasia, but this was lost in HGPIN and cancer. We conclude that MAGI-2 immunoreactivity is elevated in prostate cancer and HGPIN compared with normal tissue, and suggest that MAGI-2 may contribute to prostate carcinogenesis. This is the first report of MAGI-2 staining by immunohistochemistry in prostate cancer.

KW - Image analysis

KW - Immunohistochemistry

KW - MAGI-2

KW - Prostate cancer

KW - PTEN

UR - http://www.scopus.com/inward/record.url?scp=84962130155&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84962130155&partnerID=8YFLogxK

U2 - 10.1016/j.humpath.2016.01.003

DO - 10.1016/j.humpath.2016.01.003

M3 - Article

VL - 52

SP - 83

EP - 91

JO - Human Pathology

JF - Human Pathology

SN - 0046-8177

ER -