Macrophages in vaginal but not intestinal mucosa are monocyte-like and permissive to human immunodeficiency virus type 1 infection

Ruizhong Shen, Holly E. Richter, Ronald H. Clements, Lea Novak, Kayci Huff, Diane Bimczok, Sumathi Sankaran-Walters, Satya Dandekar, Paul R. Clapham, Lesley E. Smythies, Phillip D. Smith

Research output: Contribution to journalArticle

115 Citations (Scopus)

Abstract

Mucosal surfaces play a major role in human immunodeficiency virus type 1 (HTV-1) transmission and pathogenesis, and yet the role of lamina propria macrophages in mucosal HTV-1 infection has received little investigative attention. We report here that vaginal and intestinal macrophages display distinct phenotype and HTV-1 permissiveness profiles. Vaginal macrophages expressed the innate response receptors CD14, CD89, CD16, CD32, and CD64 and the HIV-1 receptor/coreceptors CD4, CCR5, and CXCR4, similar to monocytes. Consistent with this phenotype, green fluorescent protein-tagged R5 HIV-1 entered macrophages in explanted vaginal mucosa as early as 30 min after inoculation of virus onto the epithelium, and purified vaginal macrophages supported substantial levels of HIV-1 replication by a panel of highly macrophage-tropic R5 viruses. In sharp contrast, intestinal macrophages expressed no detectable, or very low levels of, innate response receptors and HIV-1 receptor/coreceptors and did not support HIV-1 replication, although virus occasionally entered macrophages in intestinal tissue explants. Thus, vaginal, but not intestinal, macrophages are monocyte-like and permissive to R5 HIV-1 after the virus has translocated across the epithelium. These findings suggest that genital and gut macrophages have different roles in mucosal HTV-1 pathogenesis and that vaginal macrophages play a previously underappreciated but potentially important role in mucosal HTV-1 infection in the female genital tract.

Original languageEnglish (US)
Pages (from-to)3258-3267
Number of pages10
JournalJournal of Virology
Volume83
Issue number7
DOIs
StatePublished - Apr 2009

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Virus Diseases
Intestinal Mucosa
intestinal mucosa
Human immunodeficiency virus 1
monocytes
HIV-1
Monocytes
macrophages
Macrophages
infection
HIV Receptors
receptors
Viruses
viruses
Mucous Membrane
epithelium
pathogenesis
Epithelium
vaginal mucosa
Permissiveness

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Shen, R., Richter, H. E., Clements, R. H., Novak, L., Huff, K., Bimczok, D., ... Smith, P. D. (2009). Macrophages in vaginal but not intestinal mucosa are monocyte-like and permissive to human immunodeficiency virus type 1 infection. Journal of Virology, 83(7), 3258-3267. https://doi.org/10.1128/JVI.01796-08

Macrophages in vaginal but not intestinal mucosa are monocyte-like and permissive to human immunodeficiency virus type 1 infection. / Shen, Ruizhong; Richter, Holly E.; Clements, Ronald H.; Novak, Lea; Huff, Kayci; Bimczok, Diane; Sankaran-Walters, Sumathi; Dandekar, Satya; Clapham, Paul R.; Smythies, Lesley E.; Smith, Phillip D.

In: Journal of Virology, Vol. 83, No. 7, 04.2009, p. 3258-3267.

Research output: Contribution to journalArticle

Shen, R, Richter, HE, Clements, RH, Novak, L, Huff, K, Bimczok, D, Sankaran-Walters, S, Dandekar, S, Clapham, PR, Smythies, LE & Smith, PD 2009, 'Macrophages in vaginal but not intestinal mucosa are monocyte-like and permissive to human immunodeficiency virus type 1 infection', Journal of Virology, vol. 83, no. 7, pp. 3258-3267. https://doi.org/10.1128/JVI.01796-08
Shen, Ruizhong ; Richter, Holly E. ; Clements, Ronald H. ; Novak, Lea ; Huff, Kayci ; Bimczok, Diane ; Sankaran-Walters, Sumathi ; Dandekar, Satya ; Clapham, Paul R. ; Smythies, Lesley E. ; Smith, Phillip D. / Macrophages in vaginal but not intestinal mucosa are monocyte-like and permissive to human immunodeficiency virus type 1 infection. In: Journal of Virology. 2009 ; Vol. 83, No. 7. pp. 3258-3267.
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