Macrophage Activation and Polarization: Nomenclature and Experimental Guidelines

Peter J. Murray, Judith E. Allen, Subhra K. Biswas, Edward A. Fisher, Derek W. Gilroy, Sergij Goerdt, Siamon Gordon, John A. Hamilton, Lionel B. Ivashkiv, Toby Lawrence, Massimo Locati, Alberto Mantovani, Fernando O. Martinez, Jean Louis Mege, David M. Mosser, Gioacchino Natoli, Jeroen Saeij, Joachim L. Schultze, Kari Ann Shirey, Antonio SicaJill Suttles, Irina Udalova, Jo A. vanGinderachter, Stefanie N. Vogel, Thomas A. Wynn

Research output: Contribution to journalReview article

1796 Citations (Scopus)

Abstract

Description of macrophage activation is currently contentious and confusing. Like the biblical Tower of Babel, macrophage activation encompasses a panoply of descriptors used in different ways. The lack of consensus on how to define macrophage activation in experiments invitro and invivo impedes progress in multiple ways, including the fact that many researchers still consider there to be only two types of activated macrophages, often termed M1 and M2. Here, we describe a set of standards encompassing three principles-the source of macrophages, definition of the activators, and a consensus collection of markers to describe macrophage activation-with the goal of unifying experimental standards for diverse experimental scenarios. Collectively, we propose a common framework for macrophage-activation nomenclature. The description of macrophage activation status is contentious and confusing. Murray etal. propose a framework for macrophage-activation nomenclature.

Original languageEnglish (US)
Pages (from-to)14-20
Number of pages7
JournalImmunity
Volume41
Issue number1
DOIs
StatePublished - Jul 17 2014
Externally publishedYes

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Macrophage Activation
Terminology
Guidelines
Macrophages
Research Personnel

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Medicine(all)
  • Infectious Diseases

Cite this

Murray, P. J., Allen, J. E., Biswas, S. K., Fisher, E. A., Gilroy, D. W., Goerdt, S., ... Wynn, T. A. (2014). Macrophage Activation and Polarization: Nomenclature and Experimental Guidelines. Immunity, 41(1), 14-20. https://doi.org/10.1016/j.immuni.2014.06.008

Macrophage Activation and Polarization : Nomenclature and Experimental Guidelines. / Murray, Peter J.; Allen, Judith E.; Biswas, Subhra K.; Fisher, Edward A.; Gilroy, Derek W.; Goerdt, Sergij; Gordon, Siamon; Hamilton, John A.; Ivashkiv, Lionel B.; Lawrence, Toby; Locati, Massimo; Mantovani, Alberto; Martinez, Fernando O.; Mege, Jean Louis; Mosser, David M.; Natoli, Gioacchino; Saeij, Jeroen; Schultze, Joachim L.; Shirey, Kari Ann; Sica, Antonio; Suttles, Jill; Udalova, Irina; vanGinderachter, Jo A.; Vogel, Stefanie N.; Wynn, Thomas A.

In: Immunity, Vol. 41, No. 1, 17.07.2014, p. 14-20.

Research output: Contribution to journalReview article

Murray, PJ, Allen, JE, Biswas, SK, Fisher, EA, Gilroy, DW, Goerdt, S, Gordon, S, Hamilton, JA, Ivashkiv, LB, Lawrence, T, Locati, M, Mantovani, A, Martinez, FO, Mege, JL, Mosser, DM, Natoli, G, Saeij, J, Schultze, JL, Shirey, KA, Sica, A, Suttles, J, Udalova, I, vanGinderachter, JA, Vogel, SN & Wynn, TA 2014, 'Macrophage Activation and Polarization: Nomenclature and Experimental Guidelines', Immunity, vol. 41, no. 1, pp. 14-20. https://doi.org/10.1016/j.immuni.2014.06.008
Murray PJ, Allen JE, Biswas SK, Fisher EA, Gilroy DW, Goerdt S et al. Macrophage Activation and Polarization: Nomenclature and Experimental Guidelines. Immunity. 2014 Jul 17;41(1):14-20. https://doi.org/10.1016/j.immuni.2014.06.008
Murray, Peter J. ; Allen, Judith E. ; Biswas, Subhra K. ; Fisher, Edward A. ; Gilroy, Derek W. ; Goerdt, Sergij ; Gordon, Siamon ; Hamilton, John A. ; Ivashkiv, Lionel B. ; Lawrence, Toby ; Locati, Massimo ; Mantovani, Alberto ; Martinez, Fernando O. ; Mege, Jean Louis ; Mosser, David M. ; Natoli, Gioacchino ; Saeij, Jeroen ; Schultze, Joachim L. ; Shirey, Kari Ann ; Sica, Antonio ; Suttles, Jill ; Udalova, Irina ; vanGinderachter, Jo A. ; Vogel, Stefanie N. ; Wynn, Thomas A. / Macrophage Activation and Polarization : Nomenclature and Experimental Guidelines. In: Immunity. 2014 ; Vol. 41, No. 1. pp. 14-20.
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