Macromolecular scaffolds for immobilizing small molecule microarrays in label-free detection of protein-ligand interactions on solid support

Y. S. Sun, J. P. Landry, Y. Y. Fei, X. D. Zhu, J. T. Luo, X. B. Wang, Kit Lam

Research output: Contribution to journalArticle

34 Scopus citations

Abstract

We explored two macromolecular scaffolds, bovine serum albumin (BSA) and polyvinyl alcohol (PVA), as chemically complementary platforms for immobilizing small molecule compounds on functionalized glass slides. We conjugated biotin molecules to BSA and amine-derivatized PVA and subsequently immobilized the conjugates on epoxy-functionalized glass slides through reaction of free amine residues on BSA and PVA with surface-bound epoxy groups. We studied binding reactions of such immobilized small molecule targets with solution-phase protein probes using an oblique-incidence reflectivity difference scanning optical microscope. The results showed that both BSA and amine-derivatized PVA were effective and efficient as carriers of small molecules with NHS residues and fluoric residues and for immobilization on epoxy-coated solid surfaces. A significant fraction of the conjugated small molecules retain their innate chemical activity.

Original languageEnglish (US)
Pages (from-to)5373-5380
Number of pages8
JournalAnalytical Chemistry
Volume81
Issue number13
DOIs
StatePublished - Jul 1 2009

ASJC Scopus subject areas

  • Analytical Chemistry

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