Abstract
The presence of stratum corneum carbonyls may serve as an intrinsic dosimeter for environmental oxidative damage to skin. To investigate the accumulation of carbonyls in human stratum corneum, skin was tape-stripped, then tapes were sequentially incubated with 2,4-dinitrophenyl hydrazine (DNPH), rat anti-DNP, mouse anti-rat IgG conjugated with alkaline phosphatase, p-nitrophenyl phosphate and absorbance (405 nm) measured and carbonyls estimated. Stratum corneum exposed in vitro to oxidants: hypochlorous acid (1, 10, 100 mM, ozone (0, 1, 5, 10 ppm for 2 h) or UV light (280-400 nm; 0, 4, 88, or 24 J/cm2) contained increased carbonyls. Furthermore, stratum corneum carbonyls mere elevated in tanned compared with untanned sites: dorsal hand (0.43 ± 0.06 nmol/m2) vs. lower arm (0.32 ± 0.04, mean ± S.E.M., n = 11; P < 0.003) and lower hack (0.26 ± 0.02) vs, buttock (0.21 ± 0.02; n = 6, P < 0.01) indicating in vivo oxidative damage.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 403-406 |
| Number of pages | 4 |
| Journal | FEBS Letters |
| Volume | 422 |
| Issue number | 3 |
| DOIs | |
| State | Published - Feb 6 1998 |
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Keywords
- Carbonyl
- Oxidation stress
- Ozone
- Skin
- Ultraviolet light
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Macromolecular carbonyls in human stratum corneum : A biomarker for environmental oxidant exposure? / Thiele, Jens J.; Traber, Maret G.; Re, Roberta; Espuno, Nathalie; Yan, Liang Jun; Cross, Carroll E; Packer, Lester.
In: FEBS Letters, Vol. 422, No. 3, 06.02.1998, p. 403-406.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Macromolecular carbonyls in human stratum corneum
T2 - A biomarker for environmental oxidant exposure?
AU - Thiele, Jens J.
AU - Traber, Maret G.
AU - Re, Roberta
AU - Espuno, Nathalie
AU - Yan, Liang Jun
AU - Cross, Carroll E
AU - Packer, Lester
PY - 1998/2/6
Y1 - 1998/2/6
N2 - The presence of stratum corneum carbonyls may serve as an intrinsic dosimeter for environmental oxidative damage to skin. To investigate the accumulation of carbonyls in human stratum corneum, skin was tape-stripped, then tapes were sequentially incubated with 2,4-dinitrophenyl hydrazine (DNPH), rat anti-DNP, mouse anti-rat IgG conjugated with alkaline phosphatase, p-nitrophenyl phosphate and absorbance (405 nm) measured and carbonyls estimated. Stratum corneum exposed in vitro to oxidants: hypochlorous acid (1, 10, 100 mM, ozone (0, 1, 5, 10 ppm for 2 h) or UV light (280-400 nm; 0, 4, 88, or 24 J/cm2) contained increased carbonyls. Furthermore, stratum corneum carbonyls mere elevated in tanned compared with untanned sites: dorsal hand (0.43 ± 0.06 nmol/m2) vs. lower arm (0.32 ± 0.04, mean ± S.E.M., n = 11; P < 0.003) and lower hack (0.26 ± 0.02) vs, buttock (0.21 ± 0.02; n = 6, P < 0.01) indicating in vivo oxidative damage.
AB - The presence of stratum corneum carbonyls may serve as an intrinsic dosimeter for environmental oxidative damage to skin. To investigate the accumulation of carbonyls in human stratum corneum, skin was tape-stripped, then tapes were sequentially incubated with 2,4-dinitrophenyl hydrazine (DNPH), rat anti-DNP, mouse anti-rat IgG conjugated with alkaline phosphatase, p-nitrophenyl phosphate and absorbance (405 nm) measured and carbonyls estimated. Stratum corneum exposed in vitro to oxidants: hypochlorous acid (1, 10, 100 mM, ozone (0, 1, 5, 10 ppm for 2 h) or UV light (280-400 nm; 0, 4, 88, or 24 J/cm2) contained increased carbonyls. Furthermore, stratum corneum carbonyls mere elevated in tanned compared with untanned sites: dorsal hand (0.43 ± 0.06 nmol/m2) vs. lower arm (0.32 ± 0.04, mean ± S.E.M., n = 11; P < 0.003) and lower hack (0.26 ± 0.02) vs, buttock (0.21 ± 0.02; n = 6, P < 0.01) indicating in vivo oxidative damage.
KW - Carbonyl
KW - Oxidation stress
KW - Ozone
KW - Skin
KW - Ultraviolet light
UR - http://www.scopus.com/inward/record.url?scp=0032488798&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032488798&partnerID=8YFLogxK
U2 - 10.1016/S0014-5793(98)00049-0
DO - 10.1016/S0014-5793(98)00049-0
M3 - Article
C2 - 9498826
AN - SCOPUS:0032488798
VL - 422
SP - 403
EP - 406
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 3
ER -