Macromolecular carbonyls in human stratum corneum: A biomarker for environmental oxidant exposure?

Jens J. Thiele, Maret G. Traber, Roberta Re, Nathalie Espuno, Liang Jun Yan, Carroll E Cross, Lester Packer

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

The presence of stratum corneum carbonyls may serve as an intrinsic dosimeter for environmental oxidative damage to skin. To investigate the accumulation of carbonyls in human stratum corneum, skin was tape-stripped, then tapes were sequentially incubated with 2,4-dinitrophenyl hydrazine (DNPH), rat anti-DNP, mouse anti-rat IgG conjugated with alkaline phosphatase, p-nitrophenyl phosphate and absorbance (405 nm) measured and carbonyls estimated. Stratum corneum exposed in vitro to oxidants: hypochlorous acid (1, 10, 100 mM, ozone (0, 1, 5, 10 ppm for 2 h) or UV light (280-400 nm; 0, 4, 88, or 24 J/cm2) contained increased carbonyls. Furthermore, stratum corneum carbonyls mere elevated in tanned compared with untanned sites: dorsal hand (0.43 ± 0.06 nmol/m2) vs. lower arm (0.32 ± 0.04, mean ± S.E.M., n = 11; P < 0.003) and lower hack (0.26 ± 0.02) vs, buttock (0.21 ± 0.02; n = 6, P < 0.01) indicating in vivo oxidative damage.

Original languageEnglish (US)
Pages (from-to)403-406
Number of pages4
JournalFEBS Letters
Volume422
Issue number3
DOIs
StatePublished - Feb 6 1998

Fingerprint

hydrazine
Environmental Exposure
Biomarkers
Oxidants
Cornea
Tapes
Rats
Skin
Hypochlorous Acid
Dosimeters
Ozone
Ultraviolet radiation
Alkaline Phosphatase
Surgical Tape
Immunoglobulin G
Scanning electron microscopy
Buttocks
Ultraviolet Rays
Arm
Hand

Keywords

  • Carbonyl
  • Oxidation stress
  • Ozone
  • Skin
  • Ultraviolet light

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Macromolecular carbonyls in human stratum corneum : A biomarker for environmental oxidant exposure? / Thiele, Jens J.; Traber, Maret G.; Re, Roberta; Espuno, Nathalie; Yan, Liang Jun; Cross, Carroll E; Packer, Lester.

In: FEBS Letters, Vol. 422, No. 3, 06.02.1998, p. 403-406.

Research output: Contribution to journalArticle

Thiele, Jens J. ; Traber, Maret G. ; Re, Roberta ; Espuno, Nathalie ; Yan, Liang Jun ; Cross, Carroll E ; Packer, Lester. / Macromolecular carbonyls in human stratum corneum : A biomarker for environmental oxidant exposure?. In: FEBS Letters. 1998 ; Vol. 422, No. 3. pp. 403-406.
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