Abstract
In human and non-human primates, migratory trophoblasts penetrate the uterine epithelium, invade the endometrium, enter the uterine vasculature, and migrate within the arteries. The mechanisms that regulate this directional migration are unknown. We have used early gestation macaque trophoblasts to test the hypothesis that trophoblast migration is regulated by the chemokine, Regulated on Activation T-Cell Expressed and Secreted (RANTES). Immunohistochemical analysis of cryosections of endometrial tissue showed expression of RANTES by stromal cells and vascular cells. Isolated endothelial cells expressed RANTES as determined by immunocytochemistry and RT-PCR analyses. Immunohistochemical analysis of endometrial cryosections showed that the RANTES receptor, CCR5, was expressed by trophoblasts on anchoring villi and by cells within the trophoblastic shell. Cytokeratin-positive/CCR5-positive cells, consistent with trophoblasts, were also found scattered within the stroma and were often clustered around blood vessels. Isolated trophoblast cells expressed CCR5 as determined by immunocytochemistry and RT-PCR analyses. Isolated trophoblasts migrated towards RANTES when cultured in migration chambers and migration was reduced in the presence of anti-CCR5 antibody. When trophoblasts were cultured on dishes coated with recombinant RANTES, expression of β1 integrin was increased. The RANTES-induced increase in β1 integrin expression was inhibited by pertussis toxin. These data suggest a role for RANTES and CCR5 in the regulation of trophoblast migration within the endometrium and within the uterine vasculature.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 355-364 |
| Number of pages | 10 |
| Journal | Experimental Cell Research |
| Volume | 305 |
| Issue number | 2 |
| DOIs | |
| State | Published - May 1 2005 |
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Keywords
- Chemokine
- Placenta
- Pregnancy
ASJC Scopus subject areas
- Cell Biology
Cite this
Macaque trophoblast migration is regulated by RANTES. / Thirkill, Twanda L.; Lowe, Kimberly; Vedagiri, Hemamalini; Blankenship, Thomas N.; Barakat, Abdul I.; Douglas, Gordon C.
In: Experimental Cell Research, Vol. 305, No. 2, 01.05.2005, p. 355-364.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Macaque trophoblast migration is regulated by RANTES
AU - Thirkill, Twanda L.
AU - Lowe, Kimberly
AU - Vedagiri, Hemamalini
AU - Blankenship, Thomas N.
AU - Barakat, Abdul I.
AU - Douglas, Gordon C
PY - 2005/5/1
Y1 - 2005/5/1
N2 - In human and non-human primates, migratory trophoblasts penetrate the uterine epithelium, invade the endometrium, enter the uterine vasculature, and migrate within the arteries. The mechanisms that regulate this directional migration are unknown. We have used early gestation macaque trophoblasts to test the hypothesis that trophoblast migration is regulated by the chemokine, Regulated on Activation T-Cell Expressed and Secreted (RANTES). Immunohistochemical analysis of cryosections of endometrial tissue showed expression of RANTES by stromal cells and vascular cells. Isolated endothelial cells expressed RANTES as determined by immunocytochemistry and RT-PCR analyses. Immunohistochemical analysis of endometrial cryosections showed that the RANTES receptor, CCR5, was expressed by trophoblasts on anchoring villi and by cells within the trophoblastic shell. Cytokeratin-positive/CCR5-positive cells, consistent with trophoblasts, were also found scattered within the stroma and were often clustered around blood vessels. Isolated trophoblast cells expressed CCR5 as determined by immunocytochemistry and RT-PCR analyses. Isolated trophoblasts migrated towards RANTES when cultured in migration chambers and migration was reduced in the presence of anti-CCR5 antibody. When trophoblasts were cultured on dishes coated with recombinant RANTES, expression of β1 integrin was increased. The RANTES-induced increase in β1 integrin expression was inhibited by pertussis toxin. These data suggest a role for RANTES and CCR5 in the regulation of trophoblast migration within the endometrium and within the uterine vasculature.
AB - In human and non-human primates, migratory trophoblasts penetrate the uterine epithelium, invade the endometrium, enter the uterine vasculature, and migrate within the arteries. The mechanisms that regulate this directional migration are unknown. We have used early gestation macaque trophoblasts to test the hypothesis that trophoblast migration is regulated by the chemokine, Regulated on Activation T-Cell Expressed and Secreted (RANTES). Immunohistochemical analysis of cryosections of endometrial tissue showed expression of RANTES by stromal cells and vascular cells. Isolated endothelial cells expressed RANTES as determined by immunocytochemistry and RT-PCR analyses. Immunohistochemical analysis of endometrial cryosections showed that the RANTES receptor, CCR5, was expressed by trophoblasts on anchoring villi and by cells within the trophoblastic shell. Cytokeratin-positive/CCR5-positive cells, consistent with trophoblasts, were also found scattered within the stroma and were often clustered around blood vessels. Isolated trophoblast cells expressed CCR5 as determined by immunocytochemistry and RT-PCR analyses. Isolated trophoblasts migrated towards RANTES when cultured in migration chambers and migration was reduced in the presence of anti-CCR5 antibody. When trophoblasts were cultured on dishes coated with recombinant RANTES, expression of β1 integrin was increased. The RANTES-induced increase in β1 integrin expression was inhibited by pertussis toxin. These data suggest a role for RANTES and CCR5 in the regulation of trophoblast migration within the endometrium and within the uterine vasculature.
KW - Chemokine
KW - Placenta
KW - Pregnancy
UR - http://www.scopus.com/inward/record.url?scp=16244410091&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=16244410091&partnerID=8YFLogxK
U2 - 10.1016/j.yexcr.2005.01.013
DO - 10.1016/j.yexcr.2005.01.013
M3 - Article
C2 - 15817160
AN - SCOPUS:16244410091
VL - 305
SP - 355
EP - 364
JO - Experimental Cell Research
JF - Experimental Cell Research
SN - 0014-4827
IS - 2
ER -