Macaque sperm fusion with zona-free hamster oocytes

C. A. VandeVoort, J. W. Overstreet

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Semen from six cynomolgus macaques was washed twice by centrifugation in BWW media and resuspended in 1:2 (vol:vol) of Tes-Tris-buffered eggyolk extract (EXT) diluent and BWW (EXT-BWW). Caffeine and dbcAMP were added to induce capacitation. Sperm were treated with calcium ionophore, A23187, followed by the addition of EXT to recover motility, then washed into BWW. The percent motile sperm was similar in ionophore-treated and control sperm suspensions, but motility of treated sperm decreased significantly by 20 min after treatment. The percentage of viable, acrosome-reacted sperm was 56.3% following ionophore treatment versus 4.0% in control suspensions. When ionophoretreated sperm were coincubated with zona-free hamster oocytes, 51% of oocytes had evidence of sperm fusion and no oocytes were penetrated after incubation with control sperm. These results are consistent with the hypothesis that macaque sperm are capable of fusion with zona-free hamster oocytes if sperm are viable and acrosome-reacted.

Original languageEnglish (US)
Pages (from-to)356-360
Number of pages5
JournalJournal of Medical Primatology
Volume25
Issue number5
StatePublished - Oct 1996

Keywords

  • Acrosome reaction
  • Capacitation
  • Fertilization
  • Macaca fascicularis
  • Semen

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

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  • Cite this

    VandeVoort, C. A., & Overstreet, J. W. (1996). Macaque sperm fusion with zona-free hamster oocytes. Journal of Medical Primatology, 25(5), 356-360.