Macaque sperm fusion with zona-free hamster oocytes

C. A. VandeVoort, J. W. Overstreet

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Semen from six cynomolgus macaques was washed twice by centrifugation in BWW media and resuspended in 1:2 (vol:vol) of Tes-Tris-buffered eggyolk extract (EXT) diluent and BWW (EXT-BWW). Caffeine and dbcAMP were added to induce capacitation. Sperm were treated with calcium ionophore, A23187, followed by the addition of EXT to recover motility, then washed into BWW. The percent motile sperm was similar in ionophore-treated and control sperm suspensions, but motility of treated sperm decreased significantly by 20 min after treatment. The percentage of viable, acrosome-reacted sperm was 56.3% following ionophore treatment versus 4.0% in control suspensions. When ionophoretreated sperm were coincubated with zona-free hamster oocytes, 51% of oocytes had evidence of sperm fusion and no oocytes were penetrated after incubation with control sperm. These results are consistent with the hypothesis that macaque sperm are capable of fusion with zona-free hamster oocytes if sperm are viable and acrosome-reacted.

Original languageEnglish (US)
Pages (from-to)356-360
Number of pages5
JournalJournal of Medical Primatology
Volume25
Issue number5
StatePublished - Oct 1996

Fingerprint

Herpes Zoster
Macaca
hamsters
Cricetinae
Oocytes
Spermatozoa
oocytes
spermatozoa
ionophores
Acrosome
Ionophores
acrosome
Suspensions
Calcium Ionophores
Sperm Motility
Calcimycin
Macaca fascicularis
Caffeine
Semen
Centrifugation

Keywords

  • Acrosome reaction
  • Capacitation
  • Fertilization
  • Macaca fascicularis
  • Semen

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

Cite this

VandeVoort, C. A., & Overstreet, J. W. (1996). Macaque sperm fusion with zona-free hamster oocytes. Journal of Medical Primatology, 25(5), 356-360.

Macaque sperm fusion with zona-free hamster oocytes. / VandeVoort, C. A.; Overstreet, J. W.

In: Journal of Medical Primatology, Vol. 25, No. 5, 10.1996, p. 356-360.

Research output: Contribution to journalArticle

VandeVoort, CA & Overstreet, JW 1996, 'Macaque sperm fusion with zona-free hamster oocytes', Journal of Medical Primatology, vol. 25, no. 5, pp. 356-360.
VandeVoort CA, Overstreet JW. Macaque sperm fusion with zona-free hamster oocytes. Journal of Medical Primatology. 1996 Oct;25(5):356-360.
VandeVoort, C. A. ; Overstreet, J. W. / Macaque sperm fusion with zona-free hamster oocytes. In: Journal of Medical Primatology. 1996 ; Vol. 25, No. 5. pp. 356-360.
@article{ec8aaaa338f54a15bb819fa0ee10f497,
title = "Macaque sperm fusion with zona-free hamster oocytes",
abstract = "Semen from six cynomolgus macaques was washed twice by centrifugation in BWW media and resuspended in 1:2 (vol:vol) of Tes-Tris-buffered eggyolk extract (EXT) diluent and BWW (EXT-BWW). Caffeine and dbcAMP were added to induce capacitation. Sperm were treated with calcium ionophore, A23187, followed by the addition of EXT to recover motility, then washed into BWW. The percent motile sperm was similar in ionophore-treated and control sperm suspensions, but motility of treated sperm decreased significantly by 20 min after treatment. The percentage of viable, acrosome-reacted sperm was 56.3{\%} following ionophore treatment versus 4.0{\%} in control suspensions. When ionophoretreated sperm were coincubated with zona-free hamster oocytes, 51{\%} of oocytes had evidence of sperm fusion and no oocytes were penetrated after incubation with control sperm. These results are consistent with the hypothesis that macaque sperm are capable of fusion with zona-free hamster oocytes if sperm are viable and acrosome-reacted.",
keywords = "Acrosome reaction, Capacitation, Fertilization, Macaca fascicularis, Semen",
author = "VandeVoort, {C. A.} and Overstreet, {J. W.}",
year = "1996",
month = "10",
language = "English (US)",
volume = "25",
pages = "356--360",
journal = "Journal of Medical Primatology",
issn = "0047-2565",
publisher = "Blackwell Munksgaard",
number = "5",

}

TY - JOUR

T1 - Macaque sperm fusion with zona-free hamster oocytes

AU - VandeVoort, C. A.

AU - Overstreet, J. W.

PY - 1996/10

Y1 - 1996/10

N2 - Semen from six cynomolgus macaques was washed twice by centrifugation in BWW media and resuspended in 1:2 (vol:vol) of Tes-Tris-buffered eggyolk extract (EXT) diluent and BWW (EXT-BWW). Caffeine and dbcAMP were added to induce capacitation. Sperm were treated with calcium ionophore, A23187, followed by the addition of EXT to recover motility, then washed into BWW. The percent motile sperm was similar in ionophore-treated and control sperm suspensions, but motility of treated sperm decreased significantly by 20 min after treatment. The percentage of viable, acrosome-reacted sperm was 56.3% following ionophore treatment versus 4.0% in control suspensions. When ionophoretreated sperm were coincubated with zona-free hamster oocytes, 51% of oocytes had evidence of sperm fusion and no oocytes were penetrated after incubation with control sperm. These results are consistent with the hypothesis that macaque sperm are capable of fusion with zona-free hamster oocytes if sperm are viable and acrosome-reacted.

AB - Semen from six cynomolgus macaques was washed twice by centrifugation in BWW media and resuspended in 1:2 (vol:vol) of Tes-Tris-buffered eggyolk extract (EXT) diluent and BWW (EXT-BWW). Caffeine and dbcAMP were added to induce capacitation. Sperm were treated with calcium ionophore, A23187, followed by the addition of EXT to recover motility, then washed into BWW. The percent motile sperm was similar in ionophore-treated and control sperm suspensions, but motility of treated sperm decreased significantly by 20 min after treatment. The percentage of viable, acrosome-reacted sperm was 56.3% following ionophore treatment versus 4.0% in control suspensions. When ionophoretreated sperm were coincubated with zona-free hamster oocytes, 51% of oocytes had evidence of sperm fusion and no oocytes were penetrated after incubation with control sperm. These results are consistent with the hypothesis that macaque sperm are capable of fusion with zona-free hamster oocytes if sperm are viable and acrosome-reacted.

KW - Acrosome reaction

KW - Capacitation

KW - Fertilization

KW - Macaca fascicularis

KW - Semen

UR - http://www.scopus.com/inward/record.url?scp=0030253356&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030253356&partnerID=8YFLogxK

M3 - Article

C2 - 9029400

AN - SCOPUS:0030253356

VL - 25

SP - 356

EP - 360

JO - Journal of Medical Primatology

JF - Journal of Medical Primatology

SN - 0047-2565

IS - 5

ER -