Abstract
Semen from six cynomolgus macaques was washed twice by centrifugation in BWW media and resuspended in 1:2 (vol:vol) of Tes-Tris-buffered eggyolk extract (EXT) diluent and BWW (EXT-BWW). Caffeine and dbcAMP were added to induce capacitation. Sperm were treated with calcium ionophore, A23187, followed by the addition of EXT to recover motility, then washed into BWW. The percent motile sperm was similar in ionophore-treated and control sperm suspensions, but motility of treated sperm decreased significantly by 20 min after treatment. The percentage of viable, acrosome-reacted sperm was 56.3% following ionophore treatment versus 4.0% in control suspensions. When ionophoretreated sperm were coincubated with zona-free hamster oocytes, 51% of oocytes had evidence of sperm fusion and no oocytes were penetrated after incubation with control sperm. These results are consistent with the hypothesis that macaque sperm are capable of fusion with zona-free hamster oocytes if sperm are viable and acrosome-reacted.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 356-360 |
| Number of pages | 5 |
| Journal | Journal of Medical Primatology |
| Volume | 25 |
| Issue number | 5 |
| State | Published - Oct 1996 |
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Keywords
- Acrosome reaction
- Capacitation
- Fertilization
- Macaca fascicularis
- Semen
ASJC Scopus subject areas
- Animal Science and Zoology
- veterinary(all)
Cite this
Macaque sperm fusion with zona-free hamster oocytes. / VandeVoort, C. A.; Overstreet, J. W.
In: Journal of Medical Primatology, Vol. 25, No. 5, 10.1996, p. 356-360.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Macaque sperm fusion with zona-free hamster oocytes
AU - VandeVoort, C. A.
AU - Overstreet, J. W.
PY - 1996/10
Y1 - 1996/10
N2 - Semen from six cynomolgus macaques was washed twice by centrifugation in BWW media and resuspended in 1:2 (vol:vol) of Tes-Tris-buffered eggyolk extract (EXT) diluent and BWW (EXT-BWW). Caffeine and dbcAMP were added to induce capacitation. Sperm were treated with calcium ionophore, A23187, followed by the addition of EXT to recover motility, then washed into BWW. The percent motile sperm was similar in ionophore-treated and control sperm suspensions, but motility of treated sperm decreased significantly by 20 min after treatment. The percentage of viable, acrosome-reacted sperm was 56.3% following ionophore treatment versus 4.0% in control suspensions. When ionophoretreated sperm were coincubated with zona-free hamster oocytes, 51% of oocytes had evidence of sperm fusion and no oocytes were penetrated after incubation with control sperm. These results are consistent with the hypothesis that macaque sperm are capable of fusion with zona-free hamster oocytes if sperm are viable and acrosome-reacted.
AB - Semen from six cynomolgus macaques was washed twice by centrifugation in BWW media and resuspended in 1:2 (vol:vol) of Tes-Tris-buffered eggyolk extract (EXT) diluent and BWW (EXT-BWW). Caffeine and dbcAMP were added to induce capacitation. Sperm were treated with calcium ionophore, A23187, followed by the addition of EXT to recover motility, then washed into BWW. The percent motile sperm was similar in ionophore-treated and control sperm suspensions, but motility of treated sperm decreased significantly by 20 min after treatment. The percentage of viable, acrosome-reacted sperm was 56.3% following ionophore treatment versus 4.0% in control suspensions. When ionophoretreated sperm were coincubated with zona-free hamster oocytes, 51% of oocytes had evidence of sperm fusion and no oocytes were penetrated after incubation with control sperm. These results are consistent with the hypothesis that macaque sperm are capable of fusion with zona-free hamster oocytes if sperm are viable and acrosome-reacted.
KW - Acrosome reaction
KW - Capacitation
KW - Fertilization
KW - Macaca fascicularis
KW - Semen
UR - http://www.scopus.com/inward/record.url?scp=0030253356&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030253356&partnerID=8YFLogxK
M3 - Article
C2 - 9029400
AN - SCOPUS:0030253356
VL - 25
SP - 356
EP - 360
JO - Journal of Medical Primatology
JF - Journal of Medical Primatology
SN - 0047-2565
IS - 5
ER -