M13 bacteriophage and pUC plasmids containing DNA inserts but still capable of β-galactosidase α-complementation

T. J. Close, J. L. Christmann, R. L. Rodriguez

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

A DNA fragment encoding the transposen Tn9 chloramphenicol acetyltransferase gene (cat) was inserted into M13 phage and pUC plasmid cloning vehicles. When the cat gene was inserted in the same orientation as the lacZ gene, two new polypeptides were produced. One polypeptide possessed chloramphenicol acetyltransferase activity, while the other expressed β-galactosidase α-donor activity. Both new polypeptides were translated from a hybrid messenger RNA initiating from the lac promoter. These observations may help explain why not all inserts produce white plaques.

Original languageEnglish (US)
Pages (from-to)131-136
Number of pages6
JournalGene
Volume23
Issue number2
DOIs
StatePublished - 1983

Keywords

  • blue plaques
  • chloramphenicol acetyltransferase
  • Insertional inactivation

ASJC Scopus subject areas

  • Genetics

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