M-30 and 4HNE are sequestered in different aggresomesin the same hepatocytes

Fataneh Amidi, Barbara A. French, David Chung, Charles H. Halsted, Valentina Medici, Samuel W. French

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

M-30 and 4HNE adducts are two markers of active liver disease. M-30 is a serologic marker and 4HNE adducts are histologic markers. M-30 is a marker for apoptosis because it is a fragment of cytokeratin-18 left over from proteolysis by caspase 3. 4HNE is a marker of oxidative stress because it results from lipid peroxidation. Both markers are commonly found in nonalcoholic steatohepatitis and in alcoholic hepatitis. Liver biopsies from patients with steatohepatitis, 11 alcoholic and 11 non-alcoholics were stained for 4HNE and M-30. Almost all of the biopsies in both groups showed 4HNE- and M-30-positive aggresomes in hepatocytes. Mallory Denk bodies (MDB) stained variably positive for M-30, whereas 4HNE was present in aggresomes independent of MDBs. However, they were sometimes located in hepatocytes which also contained MDBs as shown by confocal microscopy of double stained biopsies. The results indicate that the formation of M-30 and 4HNE aggresomes occurs through different pathways of liver cell injury in both types of steatohepatitis.

Original languageEnglish (US)
Pages (from-to)296-300
Number of pages5
JournalExperimental and Molecular Pathology
Volume83
Issue number3
DOIs
StatePublished - Dec 2007
Externally publishedYes

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Keywords

  • Aggresomes
  • Caspase 3
  • Mallory Denk bodies
  • Oxidative stress
  • Steatohepatitis

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Molecular Biology
  • Pathology and Forensic Medicine

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