Abstract
M-30 and 4HNE adducts are two markers of active liver disease. M-30 is a serologic marker and 4HNE adducts are histologic markers. M-30 is a marker for apoptosis because it is a fragment of cytokeratin-18 left over from proteolysis by caspase 3. 4HNE is a marker of oxidative stress because it results from lipid peroxidation. Both markers are commonly found in nonalcoholic steatohepatitis and in alcoholic hepatitis. Liver biopsies from patients with steatohepatitis, 11 alcoholic and 11 non-alcoholics were stained for 4HNE and M-30. Almost all of the biopsies in both groups showed 4HNE- and M-30-positive aggresomes in hepatocytes. Mallory Denk bodies (MDB) stained variably positive for M-30, whereas 4HNE was present in aggresomes independent of MDBs. However, they were sometimes located in hepatocytes which also contained MDBs as shown by confocal microscopy of double stained biopsies. The results indicate that the formation of M-30 and 4HNE aggresomes occurs through different pathways of liver cell injury in both types of steatohepatitis.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 296-300 |
| Number of pages | 5 |
| Journal | Experimental and Molecular Pathology |
| Volume | 83 |
| Issue number | 3 |
| DOIs | |
| State | Published - Dec 2007 |
| Externally published | Yes |
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Keywords
- Aggresomes
- Caspase 3
- Mallory Denk bodies
- Oxidative stress
- Steatohepatitis
ASJC Scopus subject areas
- Clinical Biochemistry
- Molecular Biology
- Pathology and Forensic Medicine
Cite this
M-30 and 4HNE are sequestered in different aggresomesin the same hepatocytes. / Amidi, Fataneh; French, Barbara A.; Chung, David; Halsted, Charles H.; Medici, Valentina; French, Samuel W.
In: Experimental and Molecular Pathology, Vol. 83, No. 3, 12.2007, p. 296-300.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - M-30 and 4HNE are sequestered in different aggresomesin the same hepatocytes
AU - Amidi, Fataneh
AU - French, Barbara A.
AU - Chung, David
AU - Halsted, Charles H.
AU - Medici, Valentina
AU - French, Samuel W.
PY - 2007/12
Y1 - 2007/12
N2 - M-30 and 4HNE adducts are two markers of active liver disease. M-30 is a serologic marker and 4HNE adducts are histologic markers. M-30 is a marker for apoptosis because it is a fragment of cytokeratin-18 left over from proteolysis by caspase 3. 4HNE is a marker of oxidative stress because it results from lipid peroxidation. Both markers are commonly found in nonalcoholic steatohepatitis and in alcoholic hepatitis. Liver biopsies from patients with steatohepatitis, 11 alcoholic and 11 non-alcoholics were stained for 4HNE and M-30. Almost all of the biopsies in both groups showed 4HNE- and M-30-positive aggresomes in hepatocytes. Mallory Denk bodies (MDB) stained variably positive for M-30, whereas 4HNE was present in aggresomes independent of MDBs. However, they were sometimes located in hepatocytes which also contained MDBs as shown by confocal microscopy of double stained biopsies. The results indicate that the formation of M-30 and 4HNE aggresomes occurs through different pathways of liver cell injury in both types of steatohepatitis.
AB - M-30 and 4HNE adducts are two markers of active liver disease. M-30 is a serologic marker and 4HNE adducts are histologic markers. M-30 is a marker for apoptosis because it is a fragment of cytokeratin-18 left over from proteolysis by caspase 3. 4HNE is a marker of oxidative stress because it results from lipid peroxidation. Both markers are commonly found in nonalcoholic steatohepatitis and in alcoholic hepatitis. Liver biopsies from patients with steatohepatitis, 11 alcoholic and 11 non-alcoholics were stained for 4HNE and M-30. Almost all of the biopsies in both groups showed 4HNE- and M-30-positive aggresomes in hepatocytes. Mallory Denk bodies (MDB) stained variably positive for M-30, whereas 4HNE was present in aggresomes independent of MDBs. However, they were sometimes located in hepatocytes which also contained MDBs as shown by confocal microscopy of double stained biopsies. The results indicate that the formation of M-30 and 4HNE aggresomes occurs through different pathways of liver cell injury in both types of steatohepatitis.
KW - Aggresomes
KW - Caspase 3
KW - Mallory Denk bodies
KW - Oxidative stress
KW - Steatohepatitis
UR - http://www.scopus.com/inward/record.url?scp=36048976016&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=36048976016&partnerID=8YFLogxK
U2 - 10.1016/j.yexmp.2007.09.001
DO - 10.1016/j.yexmp.2007.09.001
M3 - Article
VL - 83
SP - 296
EP - 300
JO - Experimental and Molecular Pathology
JF - Experimental and Molecular Pathology
SN - 0014-4800
IS - 3
ER -