M-30 and 4HNE are sequestered in different aggresomesin the same hepatocytes

Fataneh Amidi; Barbara A. French; David Chung; Charles H. Halsted; Valentina Medici; Samuel W. French

doi:10.1016/j.yexmp.2007.09.001

M-30 and 4HNE are sequestered in different aggresomesin the same hepatocytes

Fataneh Amidi, Barbara A. French, David Chung, Charles H. Halsted, Valentina Medici, Samuel W. French

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

M-30 and 4HNE adducts are two markers of active liver disease. M-30 is a serologic marker and 4HNE adducts are histologic markers. M-30 is a marker for apoptosis because it is a fragment of cytokeratin-18 left over from proteolysis by caspase 3. 4HNE is a marker of oxidative stress because it results from lipid peroxidation. Both markers are commonly found in nonalcoholic steatohepatitis and in alcoholic hepatitis. Liver biopsies from patients with steatohepatitis, 11 alcoholic and 11 non-alcoholics were stained for 4HNE and M-30. Almost all of the biopsies in both groups showed 4HNE- and M-30-positive aggresomes in hepatocytes. Mallory Denk bodies (MDB) stained variably positive for M-30, whereas 4HNE was present in aggresomes independent of MDBs. However, they were sometimes located in hepatocytes which also contained MDBs as shown by confocal microscopy of double stained biopsies. The results indicate that the formation of M-30 and 4HNE aggresomes occurs through different pathways of liver cell injury in both types of steatohepatitis.

Original language	English (US)
Pages (from-to)	296-300
Number of pages	5
Journal	Experimental and Molecular Pathology
Volume	83
Issue number	3
DOIs	https://doi.org/10.1016/j.yexmp.2007.09.001
State	Published - Dec 2007
Externally published	Yes

Keywords

Aggresomes
Caspase 3
Mallory Denk bodies
Oxidative stress
Steatohepatitis

ASJC Scopus subject areas

Clinical Biochemistry
Molecular Biology
Pathology and Forensic Medicine

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title = "M-30 and 4HNE are sequestered in different aggresomesin the same hepatocytes",

abstract = "M-30 and 4HNE adducts are two markers of active liver disease. M-30 is a serologic marker and 4HNE adducts are histologic markers. M-30 is a marker for apoptosis because it is a fragment of cytokeratin-18 left over from proteolysis by caspase 3. 4HNE is a marker of oxidative stress because it results from lipid peroxidation. Both markers are commonly found in nonalcoholic steatohepatitis and in alcoholic hepatitis. Liver biopsies from patients with steatohepatitis, 11 alcoholic and 11 non-alcoholics were stained for 4HNE and M-30. Almost all of the biopsies in both groups showed 4HNE- and M-30-positive aggresomes in hepatocytes. Mallory Denk bodies (MDB) stained variably positive for M-30, whereas 4HNE was present in aggresomes independent of MDBs. However, they were sometimes located in hepatocytes which also contained MDBs as shown by confocal microscopy of double stained biopsies. The results indicate that the formation of M-30 and 4HNE aggresomes occurs through different pathways of liver cell injury in both types of steatohepatitis.",

keywords = "Aggresomes, Caspase 3, Mallory Denk bodies, Oxidative stress, Steatohepatitis",

author = "Fataneh Amidi and French, {Barbara A.} and David Chung and Halsted, {Charles H.} and Valentina Medici and French, {Samuel W.}",

year = "2007",

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T1 - M-30 and 4HNE are sequestered in different aggresomesin the same hepatocytes

AU - Amidi, Fataneh

AU - French, Barbara A.

AU - Chung, David

AU - Halsted, Charles H.

AU - Medici, Valentina

AU - French, Samuel W.

PY - 2007/12

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N2 - M-30 and 4HNE adducts are two markers of active liver disease. M-30 is a serologic marker and 4HNE adducts are histologic markers. M-30 is a marker for apoptosis because it is a fragment of cytokeratin-18 left over from proteolysis by caspase 3. 4HNE is a marker of oxidative stress because it results from lipid peroxidation. Both markers are commonly found in nonalcoholic steatohepatitis and in alcoholic hepatitis. Liver biopsies from patients with steatohepatitis, 11 alcoholic and 11 non-alcoholics were stained for 4HNE and M-30. Almost all of the biopsies in both groups showed 4HNE- and M-30-positive aggresomes in hepatocytes. Mallory Denk bodies (MDB) stained variably positive for M-30, whereas 4HNE was present in aggresomes independent of MDBs. However, they were sometimes located in hepatocytes which also contained MDBs as shown by confocal microscopy of double stained biopsies. The results indicate that the formation of M-30 and 4HNE aggresomes occurs through different pathways of liver cell injury in both types of steatohepatitis.

AB - M-30 and 4HNE adducts are two markers of active liver disease. M-30 is a serologic marker and 4HNE adducts are histologic markers. M-30 is a marker for apoptosis because it is a fragment of cytokeratin-18 left over from proteolysis by caspase 3. 4HNE is a marker of oxidative stress because it results from lipid peroxidation. Both markers are commonly found in nonalcoholic steatohepatitis and in alcoholic hepatitis. Liver biopsies from patients with steatohepatitis, 11 alcoholic and 11 non-alcoholics were stained for 4HNE and M-30. Almost all of the biopsies in both groups showed 4HNE- and M-30-positive aggresomes in hepatocytes. Mallory Denk bodies (MDB) stained variably positive for M-30, whereas 4HNE was present in aggresomes independent of MDBs. However, they were sometimes located in hepatocytes which also contained MDBs as shown by confocal microscopy of double stained biopsies. The results indicate that the formation of M-30 and 4HNE aggresomes occurs through different pathways of liver cell injury in both types of steatohepatitis.

KW - Aggresomes

KW - Caspase 3

KW - Mallory Denk bodies

KW - Oxidative stress

KW - Steatohepatitis

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