Lysyl oxidase and P-ATPase-7A expression during embryonic development in the rat

E. H. Tchaparian, J. Y. Uriu-Adams, Carl L Keen, A. E. Mitchell, R. B. Rucker

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Lysyl oxidase activity is critical for the assembly and cross-linking of extracellular matrix proteins, such as collagen and elastin. Moreover, lysyl oxidase activity is sensitive to changes in copper status and genetic perturbations in copper transport, e.g., mutations in the P-type ATPase gene, ATP7A, associated with cellular copper transport. Lysyl oxidase may also serve as a vehicle for copper transport from extracellular matrix cells. Herein, we demonstrate that sufficient lysyl oxidase functional activity is present in the rat embryo at gestation day (GD) 9 to be detected in conventional enzyme assays. Estimation of embryonic lysyl oxidase functional activity, however, required partial purification in order to remove inhibitors. From GD 9 to GD 15, lysyl oxidase activity was relatively constant when expressed per unit of protein or DNA. In contrast, the steady- state levels of lysyl oxidase and ATP7A mRNA, measured by RT-PCR and expressed relative to total RNA and cyclophilin mRNA, increased approximately fourfold from GD 9 to 15. The pattern of temporal expression for ATP7A was consistent with its possible role in copper delivery to lysyl oxidase. (C) 2000 Academic Press.

Original languageEnglish (US)
Pages (from-to)71-77
Number of pages7
JournalArchives of Biochemistry and Biophysics
Volume379
Issue number1
DOIs
StatePublished - Jul 1 2000

Fingerprint

Protein-Lysine 6-Oxidase
Embryonic Development
Adenosine Triphosphatases
Rats
Copper
Pregnancy
Cyclophilins
Messenger RNA
Elastin
Extracellular Matrix Proteins
Enzyme Assays
Purification
Extracellular Matrix
Assays
Collagen
Embryonic Structures
Genes
RNA
Polymerase Chain Reaction
Mutation

Keywords

  • And copper metabolism
  • Development
  • Lysyl oxidase
  • Menkes disease
  • P-ATPase-7A

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Tchaparian, E. H., Uriu-Adams, J. Y., Keen, C. L., Mitchell, A. E., & Rucker, R. B. (2000). Lysyl oxidase and P-ATPase-7A expression during embryonic development in the rat. Archives of Biochemistry and Biophysics, 379(1), 71-77. https://doi.org/10.1006/abbi.2000.1842

Lysyl oxidase and P-ATPase-7A expression during embryonic development in the rat. / Tchaparian, E. H.; Uriu-Adams, J. Y.; Keen, Carl L; Mitchell, A. E.; Rucker, R. B.

In: Archives of Biochemistry and Biophysics, Vol. 379, No. 1, 01.07.2000, p. 71-77.

Research output: Contribution to journalArticle

Tchaparian, EH, Uriu-Adams, JY, Keen, CL, Mitchell, AE & Rucker, RB 2000, 'Lysyl oxidase and P-ATPase-7A expression during embryonic development in the rat', Archives of Biochemistry and Biophysics, vol. 379, no. 1, pp. 71-77. https://doi.org/10.1006/abbi.2000.1842
Tchaparian, E. H. ; Uriu-Adams, J. Y. ; Keen, Carl L ; Mitchell, A. E. ; Rucker, R. B. / Lysyl oxidase and P-ATPase-7A expression during embryonic development in the rat. In: Archives of Biochemistry and Biophysics. 2000 ; Vol. 379, No. 1. pp. 71-77.
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