Lysozyme-enhanced killing of candida albicans and coccidioides immitis by amphotericin b

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12 Scopus citations

Abstract

The concentration of amphotericin B required to inhibit the growth in chemically defined media of 3 strains of Candida albicans and the spherule and mycelial growth phases of Coccidioides immitis Silveira strain was decreased 4-to-16-fold by the incorporation of 5-10 μg lysozyme /ml. Despite this enhancement of sensitivity to amphotericin B by physiological (serum) concentrations of lysozyme, the 3 C. albicans strains and the mycelial phase of C. immitis in the absence of amphotericin B were not inhibited by 1000-2000 μg lysozyme/ml. In contrast, the spherule phase of C. immitis was inhibited by 5-20 μg lysozyme/ml. Ten μg lysozyme/ml markedly but reversibly altered the characteristic absorption spectra of amphotericin B and amphotericin B- cholesterol complexes thus suggesting that the lysozyme enhanced sensitivity to amphotericin B shown in lysozyme-insensitive strains may be a consequence of lysozyme binding to cell membrane sterol-amphotericin B complexes.

Original languageEnglish (US)
Pages (from-to)329-340
Number of pages12
JournalMedical Mycology
Volume12
Issue number3
DOIs
StatePublished - 1974

ASJC Scopus subject areas

  • veterinary(all)
  • Infectious Diseases

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