Lysis of allogeneic human lymphocytes by nonspecifically activated T-like cells

A. J. McMichael, F. M. Gotch, James Hildreth

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Abstract

In the generation of cytotoxic effector cells specific for influenza A virus-infected lymphocytes, 3 donors have given an unusual pattern of lytic activity, killing HLA-mismatched target cells. This has been analyzed in detail for 1 donor and 1 of the other 2 shows similar results. Activation only requires culture in medium between 1 and 4 days and parallels development of cell line K562-directed natural killer cells. Target lymphocytes do not need to be virus-infected and appear to be normal lymphocytes. The effector cells carry the surface markers T3 and T8 defined by OKT3/anti-Leu4 and OKT8/anti-Leu2a monoclonal antibodies, respectively. Unlike HLA class 1-restricted or -directed cytotoxic T cells, neither anti-Leu2a nor anti-Leu4 blocked killing in the absence of complement. MHM23, a monoclonal antibody specific for the human lymphocyte function antigen, blocked lysis. The results indicate that these effector cells are related to cytotoxic T lymphocytes, but can lyse allogeneic target cells through a different recognition process. There is some specificity because autologous cells were not killed.

Original languageEnglish (US)
Pages (from-to)1002-1005
Number of pages4
JournalEuropean Journal of Immunology
Volume12
Issue number12
StatePublished - 1982
Externally publishedYes

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ASJC Scopus subject areas

  • Immunology

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