Lymphocyte proliferative responses to human immunodeficiency virus antigens in vitro

J. F. Krowka, D. P. Stites, Sharad Jain, K. S. Steimer, C. George-Nascimento, A. Gyenes, P. J. Barr, H. Hollander, A. R. Moss, J. M. Homsy, J. A. Levy, D. I. Abrams

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63 Scopus citations

Abstract

All HIV seronegative (HIV Ab-) and most HIV seropositive (HIV Ab+) individuals' lymphocytes failed to proliferate in primary cultures in response to purified HIV or to recombinant envelope and core antigens of HIV, even in the presence of recombinant interleukin 2 (rIL-2). Most HIV Ab- and HIV Ab+ individuals' lymphocytes, however, could proliferate or be induced by rIL-2 to proliferate in response to lysates of Escherichia coli or Saccharomyces cerevisiae. These findings indicate selective defects in lymphocyte proliferate responses to HIV antigens before the development of AIDS in which lymphocytes are unable to proliferate in response to any antigens. These defects in cell-mediated immune responses to HIV antigens are likely to play an important role in the pathobiology of HIV infections. Although intact HIV or glycosylated gp120 envelope protein of HIV are involved in these defects, a non-glycosylated recombinant form of the HIV gp120 envelope (ENV2-3) and p25 core proteins did not inhibit antigen- or mitogen-driven lymphocyte proliferation.

Original languageEnglish (US)
Pages (from-to)1198-1203
Number of pages6
JournalJournal of Clinical Investigation
Volume83
Issue number4
DOIs
StatePublished - Jan 1 1989
Externally publishedYes

ASJC Scopus subject areas

  • Medicine(all)

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    Krowka, J. F., Stites, D. P., Jain, S., Steimer, K. S., George-Nascimento, C., Gyenes, A., Barr, P. J., Hollander, H., Moss, A. R., Homsy, J. M., Levy, J. A., & Abrams, D. I. (1989). Lymphocyte proliferative responses to human immunodeficiency virus antigens in vitro. Journal of Clinical Investigation, 83(4), 1198-1203. https://doi.org/10.1172/JCI114001