Lymphocyte proliferative responses to human immunodeficiency virus antigens in vitro

J. F. Krowka, D. P. Stites, Sharad Jain, K. S. Steimer, C. George-Nascimento, A. Gyenes, P. J. Barr, H. Hollander, A. R. Moss, J. M. Homsy, J. A. Levy, D. I. Abrams

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

All HIV seronegative (HIV Ab-) and most HIV seropositive (HIV Ab+) individuals' lymphocytes failed to proliferate in primary cultures in response to purified HIV or to recombinant envelope and core antigens of HIV, even in the presence of recombinant interleukin 2 (rIL-2). Most HIV Ab- and HIV Ab+ individuals' lymphocytes, however, could proliferate or be induced by rIL-2 to proliferate in response to lysates of Escherichia coli or Saccharomyces cerevisiae. These findings indicate selective defects in lymphocyte proliferate responses to HIV antigens before the development of AIDS in which lymphocytes are unable to proliferate in response to any antigens. These defects in cell-mediated immune responses to HIV antigens are likely to play an important role in the pathobiology of HIV infections. Although intact HIV or glycosylated gp120 envelope protein of HIV are involved in these defects, a non-glycosylated recombinant form of the HIV gp120 envelope (ENV2-3) and p25 core proteins did not inhibit antigen- or mitogen-driven lymphocyte proliferation.

Original languageEnglish (US)
Pages (from-to)1198-1203
Number of pages6
JournalJournal of Clinical Investigation
Volume83
Issue number4
DOIs
StatePublished - Jan 1 1989
Externally publishedYes

Fingerprint

HIV
Lymphocytes
Antigens
HIV Antigens
HIV Envelope Protein gp120
Interleukin-2
In Vitro Techniques
Mitogens
HIV Infections
Saccharomyces cerevisiae
Acquired Immunodeficiency Syndrome
Escherichia coli
Proteins

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Krowka, J. F., Stites, D. P., Jain, S., Steimer, K. S., George-Nascimento, C., Gyenes, A., ... Abrams, D. I. (1989). Lymphocyte proliferative responses to human immunodeficiency virus antigens in vitro. Journal of Clinical Investigation, 83(4), 1198-1203. https://doi.org/10.1172/JCI114001

Lymphocyte proliferative responses to human immunodeficiency virus antigens in vitro. / Krowka, J. F.; Stites, D. P.; Jain, Sharad; Steimer, K. S.; George-Nascimento, C.; Gyenes, A.; Barr, P. J.; Hollander, H.; Moss, A. R.; Homsy, J. M.; Levy, J. A.; Abrams, D. I.

In: Journal of Clinical Investigation, Vol. 83, No. 4, 01.01.1989, p. 1198-1203.

Research output: Contribution to journalArticle

Krowka, JF, Stites, DP, Jain, S, Steimer, KS, George-Nascimento, C, Gyenes, A, Barr, PJ, Hollander, H, Moss, AR, Homsy, JM, Levy, JA & Abrams, DI 1989, 'Lymphocyte proliferative responses to human immunodeficiency virus antigens in vitro', Journal of Clinical Investigation, vol. 83, no. 4, pp. 1198-1203. https://doi.org/10.1172/JCI114001
Krowka, J. F. ; Stites, D. P. ; Jain, Sharad ; Steimer, K. S. ; George-Nascimento, C. ; Gyenes, A. ; Barr, P. J. ; Hollander, H. ; Moss, A. R. ; Homsy, J. M. ; Levy, J. A. ; Abrams, D. I. / Lymphocyte proliferative responses to human immunodeficiency virus antigens in vitro. In: Journal of Clinical Investigation. 1989 ; Vol. 83, No. 4. pp. 1198-1203.
@article{17a7bca653724dfab5b9e978a246c733,
title = "Lymphocyte proliferative responses to human immunodeficiency virus antigens in vitro",
abstract = "All HIV seronegative (HIV Ab-) and most HIV seropositive (HIV Ab+) individuals' lymphocytes failed to proliferate in primary cultures in response to purified HIV or to recombinant envelope and core antigens of HIV, even in the presence of recombinant interleukin 2 (rIL-2). Most HIV Ab- and HIV Ab+ individuals' lymphocytes, however, could proliferate or be induced by rIL-2 to proliferate in response to lysates of Escherichia coli or Saccharomyces cerevisiae. These findings indicate selective defects in lymphocyte proliferate responses to HIV antigens before the development of AIDS in which lymphocytes are unable to proliferate in response to any antigens. These defects in cell-mediated immune responses to HIV antigens are likely to play an important role in the pathobiology of HIV infections. Although intact HIV or glycosylated gp120 envelope protein of HIV are involved in these defects, a non-glycosylated recombinant form of the HIV gp120 envelope (ENV2-3) and p25 core proteins did not inhibit antigen- or mitogen-driven lymphocyte proliferation.",
author = "Krowka, {J. F.} and Stites, {D. P.} and Sharad Jain and Steimer, {K. S.} and C. George-Nascimento and A. Gyenes and Barr, {P. J.} and H. Hollander and Moss, {A. R.} and Homsy, {J. M.} and Levy, {J. A.} and Abrams, {D. I.}",
year = "1989",
month = "1",
day = "1",
doi = "10.1172/JCI114001",
language = "English (US)",
volume = "83",
pages = "1198--1203",
journal = "Journal of Clinical Investigation",
issn = "0021-9738",
publisher = "The American Society for Clinical Investigation",
number = "4",

}

TY - JOUR

T1 - Lymphocyte proliferative responses to human immunodeficiency virus antigens in vitro

AU - Krowka, J. F.

AU - Stites, D. P.

AU - Jain, Sharad

AU - Steimer, K. S.

AU - George-Nascimento, C.

AU - Gyenes, A.

AU - Barr, P. J.

AU - Hollander, H.

AU - Moss, A. R.

AU - Homsy, J. M.

AU - Levy, J. A.

AU - Abrams, D. I.

PY - 1989/1/1

Y1 - 1989/1/1

N2 - All HIV seronegative (HIV Ab-) and most HIV seropositive (HIV Ab+) individuals' lymphocytes failed to proliferate in primary cultures in response to purified HIV or to recombinant envelope and core antigens of HIV, even in the presence of recombinant interleukin 2 (rIL-2). Most HIV Ab- and HIV Ab+ individuals' lymphocytes, however, could proliferate or be induced by rIL-2 to proliferate in response to lysates of Escherichia coli or Saccharomyces cerevisiae. These findings indicate selective defects in lymphocyte proliferate responses to HIV antigens before the development of AIDS in which lymphocytes are unable to proliferate in response to any antigens. These defects in cell-mediated immune responses to HIV antigens are likely to play an important role in the pathobiology of HIV infections. Although intact HIV or glycosylated gp120 envelope protein of HIV are involved in these defects, a non-glycosylated recombinant form of the HIV gp120 envelope (ENV2-3) and p25 core proteins did not inhibit antigen- or mitogen-driven lymphocyte proliferation.

AB - All HIV seronegative (HIV Ab-) and most HIV seropositive (HIV Ab+) individuals' lymphocytes failed to proliferate in primary cultures in response to purified HIV or to recombinant envelope and core antigens of HIV, even in the presence of recombinant interleukin 2 (rIL-2). Most HIV Ab- and HIV Ab+ individuals' lymphocytes, however, could proliferate or be induced by rIL-2 to proliferate in response to lysates of Escherichia coli or Saccharomyces cerevisiae. These findings indicate selective defects in lymphocyte proliferate responses to HIV antigens before the development of AIDS in which lymphocytes are unable to proliferate in response to any antigens. These defects in cell-mediated immune responses to HIV antigens are likely to play an important role in the pathobiology of HIV infections. Although intact HIV or glycosylated gp120 envelope protein of HIV are involved in these defects, a non-glycosylated recombinant form of the HIV gp120 envelope (ENV2-3) and p25 core proteins did not inhibit antigen- or mitogen-driven lymphocyte proliferation.

UR - http://www.scopus.com/inward/record.url?scp=0024567058&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024567058&partnerID=8YFLogxK

U2 - 10.1172/JCI114001

DO - 10.1172/JCI114001

M3 - Article

C2 - 2703528

AN - SCOPUS:0024567058

VL - 83

SP - 1198

EP - 1203

JO - Journal of Clinical Investigation

JF - Journal of Clinical Investigation

SN - 0021-9738

IS - 4

ER -