Lymphocyte and monocyte-induced motility of MCF-7 cells by tumor necrosis factor-

Philip M. Carpenter, Tetsuya Gatanaga, Hoa Nguyen, John C. Hiserodt

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

A potentially important tumor-host interaction is increased tumor-cell invasiveness in response to motility factors derived from stromal and lymphoid cells. Conditioned medium of IL-2-stimulated lymphocytes and fractions enriched in either T cells, natural killer (NK) cells, or monocytes induced motility in MCF-7 breast carcinoma cells. ELISA and antibody neutralization studies demonstrated that this effect was due to tumor necrosis factor-α (TNF-α) secretion by the lymphoid cells or the enriched fractions. Unstimulated leukocytes in direct contact with MCF-7 cells also induced motility that was inhibited by anti-TNF-α antiserum. Time-lapse video microscopy of cells exposed to 10 ng/ml TNF-α showed that motility was independent of its toxic effects. Immunoperoxidase showed that MCF-7 cells expressed both the 55-kDa and the 75-kDa TNF-α receptors (TNFR). Antiserum against the 55-kDa TNFR, like TNF-α, induced motility in MCF-7 cells. This was most likely due to cross-linking of the 55-kDa TNFR monomers, since the monomeric F(ab) did not produce this effect. Our results raise the possibility that TNF-α-induced motility is one mechanism by which tumor cells overcome the potential anti-tumor immune function of lymphocytes and macrophages in peri-tumoral infiltrates.

Original languageEnglish (US)
Pages (from-to)64-70
Number of pages7
JournalInternational Journal of Cancer
Volume71
Issue number1
DOIs
StatePublished - Apr 23 1997

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ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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