Lyme neuroborreliosis in 2 horses

Denise Imai, B. C. Barr, B. Daft, J. J. Bertone, S. Feng, Emir Hodzic, J. M. Johnston, K. J. Olsen, Stephen W Barthold

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Lyme neuroborreliosis-characterized as chronic, necrosuppurative to nonsuppurative, perivascular to diffuse meningoradiculoneuritis-was diagnosed in 2 horses with progressive neurologic disease. In 1 horse, Borrelia burgdorferi sensu stricto was identified by polymerase chain reaction amplification of B burgdorferi sensu stricto-specific gene targets (ospA, ospC, flaB, dbpA, arp). Highest spirochetal burdens were in tissues with inflammation, including spinal cord, muscle, and joint capsule. Sequence analysis of ospA, ospC, and flaB revealed 99.9% sequence identity to the respective genes in B burgdorferi strain 297, an isolate from a human case of neuroborreliosis. In both horses, spirochetes were visualized in affected tissues with Steiner silver impregnation and by immunohistochemistry, predominantly within the dense collagenous tissue of the dura mater and leptomeninges.

Original languageEnglish (US)
Pages (from-to)1151-1157
Number of pages7
JournalVeterinary Pathology
Volume48
Issue number6
DOIs
StatePublished - Nov 29 2011

Fingerprint

Lyme Neuroborreliosis
Horses
horses
Myelitis
Joint Capsule
Dura Mater
Spirochaetales
Borrelia burgdorferi
nervous system diseases
Nervous System Diseases
Silver
spinal cord
silver
Genes
immunohistochemistry
Sequence Analysis
genes
sequence analysis
inflammation
polymerase chain reaction

Keywords

  • Borrelia burgdorferi
  • equine
  • Lyme disease
  • meningitis
  • neuroborreliosis
  • polyradiculoneuritis

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Lyme neuroborreliosis in 2 horses. / Imai, Denise; Barr, B. C.; Daft, B.; Bertone, J. J.; Feng, S.; Hodzic, Emir; Johnston, J. M.; Olsen, K. J.; Barthold, Stephen W.

In: Veterinary Pathology, Vol. 48, No. 6, 29.11.2011, p. 1151-1157.

Research output: Contribution to journalArticle

Imai, D, Barr, BC, Daft, B, Bertone, JJ, Feng, S, Hodzic, E, Johnston, JM, Olsen, KJ & Barthold, SW 2011, 'Lyme neuroborreliosis in 2 horses', Veterinary Pathology, vol. 48, no. 6, pp. 1151-1157. https://doi.org/10.1177/0300985811398246
Imai D, Barr BC, Daft B, Bertone JJ, Feng S, Hodzic E et al. Lyme neuroborreliosis in 2 horses. Veterinary Pathology. 2011 Nov 29;48(6):1151-1157. https://doi.org/10.1177/0300985811398246
Imai, Denise ; Barr, B. C. ; Daft, B. ; Bertone, J. J. ; Feng, S. ; Hodzic, Emir ; Johnston, J. M. ; Olsen, K. J. ; Barthold, Stephen W. / Lyme neuroborreliosis in 2 horses. In: Veterinary Pathology. 2011 ; Vol. 48, No. 6. pp. 1151-1157.
@article{e419ce448c1f4c098f75de469c7d04b8,
title = "Lyme neuroborreliosis in 2 horses",
abstract = "Lyme neuroborreliosis-characterized as chronic, necrosuppurative to nonsuppurative, perivascular to diffuse meningoradiculoneuritis-was diagnosed in 2 horses with progressive neurologic disease. In 1 horse, Borrelia burgdorferi sensu stricto was identified by polymerase chain reaction amplification of B burgdorferi sensu stricto-specific gene targets (ospA, ospC, flaB, dbpA, arp). Highest spirochetal burdens were in tissues with inflammation, including spinal cord, muscle, and joint capsule. Sequence analysis of ospA, ospC, and flaB revealed 99.9{\%} sequence identity to the respective genes in B burgdorferi strain 297, an isolate from a human case of neuroborreliosis. In both horses, spirochetes were visualized in affected tissues with Steiner silver impregnation and by immunohistochemistry, predominantly within the dense collagenous tissue of the dura mater and leptomeninges.",
keywords = "Borrelia burgdorferi, equine, Lyme disease, meningitis, neuroborreliosis, polyradiculoneuritis",
author = "Denise Imai and Barr, {B. C.} and B. Daft and Bertone, {J. J.} and S. Feng and Emir Hodzic and Johnston, {J. M.} and Olsen, {K. J.} and Barthold, {Stephen W}",
year = "2011",
month = "11",
day = "29",
doi = "10.1177/0300985811398246",
language = "English (US)",
volume = "48",
pages = "1151--1157",
journal = "Veterinary Pathology",
issn = "0300-9858",
publisher = "SAGE Publications Ltd",
number = "6",

}

TY - JOUR

T1 - Lyme neuroborreliosis in 2 horses

AU - Imai, Denise

AU - Barr, B. C.

AU - Daft, B.

AU - Bertone, J. J.

AU - Feng, S.

AU - Hodzic, Emir

AU - Johnston, J. M.

AU - Olsen, K. J.

AU - Barthold, Stephen W

PY - 2011/11/29

Y1 - 2011/11/29

N2 - Lyme neuroborreliosis-characterized as chronic, necrosuppurative to nonsuppurative, perivascular to diffuse meningoradiculoneuritis-was diagnosed in 2 horses with progressive neurologic disease. In 1 horse, Borrelia burgdorferi sensu stricto was identified by polymerase chain reaction amplification of B burgdorferi sensu stricto-specific gene targets (ospA, ospC, flaB, dbpA, arp). Highest spirochetal burdens were in tissues with inflammation, including spinal cord, muscle, and joint capsule. Sequence analysis of ospA, ospC, and flaB revealed 99.9% sequence identity to the respective genes in B burgdorferi strain 297, an isolate from a human case of neuroborreliosis. In both horses, spirochetes were visualized in affected tissues with Steiner silver impregnation and by immunohistochemistry, predominantly within the dense collagenous tissue of the dura mater and leptomeninges.

AB - Lyme neuroborreliosis-characterized as chronic, necrosuppurative to nonsuppurative, perivascular to diffuse meningoradiculoneuritis-was diagnosed in 2 horses with progressive neurologic disease. In 1 horse, Borrelia burgdorferi sensu stricto was identified by polymerase chain reaction amplification of B burgdorferi sensu stricto-specific gene targets (ospA, ospC, flaB, dbpA, arp). Highest spirochetal burdens were in tissues with inflammation, including spinal cord, muscle, and joint capsule. Sequence analysis of ospA, ospC, and flaB revealed 99.9% sequence identity to the respective genes in B burgdorferi strain 297, an isolate from a human case of neuroborreliosis. In both horses, spirochetes were visualized in affected tissues with Steiner silver impregnation and by immunohistochemistry, predominantly within the dense collagenous tissue of the dura mater and leptomeninges.

KW - Borrelia burgdorferi

KW - equine

KW - Lyme disease

KW - meningitis

KW - neuroborreliosis

KW - polyradiculoneuritis

UR - http://www.scopus.com/inward/record.url?scp=80052465759&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80052465759&partnerID=8YFLogxK

U2 - 10.1177/0300985811398246

DO - 10.1177/0300985811398246

M3 - Article

C2 - 21285382

AN - SCOPUS:80052465759

VL - 48

SP - 1151

EP - 1157

JO - Veterinary Pathology

JF - Veterinary Pathology

SN - 0300-9858

IS - 6

ER -