Lyme disease in transgenic mice expressing the Borrelia burgdorferi flagellin epitope implicated in human neuroborreliosis

Erol Fikrig, Stephen W Barthold

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Because of an association of human neuroborreliosis with the development of an antibody response against an antigen in neural tissue that cross-reacts with an epitope on the flagellin protein of Borrelia burgdorferi, C3H transgenic mice were created that expressed the flagellin epitope (amino acids 213-224) as a fusion protein with myelin basic protein. The transgenic mice expressed the flagellin epitope selectively in myelinated regions of the nervous system. Both transgenic and non-transgenic mice developed an antibody response to the flagellin epitope during B. burgdorferi infection and both developed arthritis and carditis. However, no lesions were found in the central nervous system of either type of mouse for up to 8 weeks after infection. The data indicate that expression of the flagellin 213-24 epitope in mice does not result in neurologic disease, suggesting that B. burgdorferi flagellin antibodies may not be directly implicated in neuroborreliosis.

Original languageEnglish (US)
Pages (from-to)137-143
Number of pages7
JournalFEMS Microbiology Letters
Volume148
Issue number2
DOIs
StatePublished - 1997
Externally publishedYes

Fingerprint

Flagellin
Borrelia burgdorferi
Lyme Disease
Transgenic Mice
Epitopes
Antibody Formation
Borrelia Infections
Myelin Basic Protein
Inbred C3H Mouse
Myocarditis
Nervous System Diseases
Nervous System
Arthritis
Proteins
Central Nervous System
Antigens
Amino Acids
Antibodies
Infection

Keywords

  • Borrelia burgdorferi
  • flagelling
  • Lyme disease
  • nervous system
  • spirochete

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

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abstract = "Because of an association of human neuroborreliosis with the development of an antibody response against an antigen in neural tissue that cross-reacts with an epitope on the flagellin protein of Borrelia burgdorferi, C3H transgenic mice were created that expressed the flagellin epitope (amino acids 213-224) as a fusion protein with myelin basic protein. The transgenic mice expressed the flagellin epitope selectively in myelinated regions of the nervous system. Both transgenic and non-transgenic mice developed an antibody response to the flagellin epitope during B. burgdorferi infection and both developed arthritis and carditis. However, no lesions were found in the central nervous system of either type of mouse for up to 8 weeks after infection. The data indicate that expression of the flagellin 213-24 epitope in mice does not result in neurologic disease, suggesting that B. burgdorferi flagellin antibodies may not be directly implicated in neuroborreliosis.",
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