Abstract
Because of an association of human neuroborreliosis with the development of an antibody response against an antigen in neural tissue that cross-reacts with an epitope on the flagellin protein of Borrelia burgdorferi, C3H transgenic mice were created that expressed the flagellin epitope (amino acids 213-224) as a fusion protein with myelin basic protein. The transgenic mice expressed the flagellin epitope selectively in myelinated regions of the nervous system. Both transgenic and non-transgenic mice developed an antibody response to the flagellin epitope during B. burgdorferi infection and both developed arthritis and carditis. However, no lesions were found in the central nervous system of either type of mouse for up to 8 weeks after infection. The data indicate that expression of the flagellin 213-24 epitope in mice does not result in neurologic disease, suggesting that B. burgdorferi flagellin antibodies may not be directly implicated in neuroborreliosis.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 137-143 |
| Number of pages | 7 |
| Journal | FEMS Microbiology Letters |
| Volume | 148 |
| Issue number | 2 |
| DOIs | |
| State | Published - 1997 |
| Externally published | Yes |
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Keywords
- Borrelia burgdorferi
- flagelling
- Lyme disease
- nervous system
- spirochete
ASJC Scopus subject areas
- Genetics
- Molecular Biology
- Applied Microbiology and Biotechnology
- Microbiology
Cite this
Lyme disease in transgenic mice expressing the Borrelia burgdorferi flagellin epitope implicated in human neuroborreliosis. / Fikrig, Erol; Barthold, Stephen W.
In: FEMS Microbiology Letters, Vol. 148, No. 2, 1997, p. 137-143.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Lyme disease in transgenic mice expressing the Borrelia burgdorferi flagellin epitope implicated in human neuroborreliosis
AU - Fikrig, Erol
AU - Barthold, Stephen W
PY - 1997
Y1 - 1997
N2 - Because of an association of human neuroborreliosis with the development of an antibody response against an antigen in neural tissue that cross-reacts with an epitope on the flagellin protein of Borrelia burgdorferi, C3H transgenic mice were created that expressed the flagellin epitope (amino acids 213-224) as a fusion protein with myelin basic protein. The transgenic mice expressed the flagellin epitope selectively in myelinated regions of the nervous system. Both transgenic and non-transgenic mice developed an antibody response to the flagellin epitope during B. burgdorferi infection and both developed arthritis and carditis. However, no lesions were found in the central nervous system of either type of mouse for up to 8 weeks after infection. The data indicate that expression of the flagellin 213-24 epitope in mice does not result in neurologic disease, suggesting that B. burgdorferi flagellin antibodies may not be directly implicated in neuroborreliosis.
AB - Because of an association of human neuroborreliosis with the development of an antibody response against an antigen in neural tissue that cross-reacts with an epitope on the flagellin protein of Borrelia burgdorferi, C3H transgenic mice were created that expressed the flagellin epitope (amino acids 213-224) as a fusion protein with myelin basic protein. The transgenic mice expressed the flagellin epitope selectively in myelinated regions of the nervous system. Both transgenic and non-transgenic mice developed an antibody response to the flagellin epitope during B. burgdorferi infection and both developed arthritis and carditis. However, no lesions were found in the central nervous system of either type of mouse for up to 8 weeks after infection. The data indicate that expression of the flagellin 213-24 epitope in mice does not result in neurologic disease, suggesting that B. burgdorferi flagellin antibodies may not be directly implicated in neuroborreliosis.
KW - Borrelia burgdorferi
KW - flagelling
KW - Lyme disease
KW - nervous system
KW - spirochete
UR - http://www.scopus.com/inward/record.url?scp=0030938442&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030938442&partnerID=8YFLogxK
U2 - 10.1016/S0378-1097(97)00020-7
DO - 10.1016/S0378-1097(97)00020-7
M3 - Article
C2 - 9084140
AN - SCOPUS:0030938442
VL - 148
SP - 137
EP - 143
JO - FEMS Microbiology Letters
JF - FEMS Microbiology Letters
SN - 0378-1097
IS - 2
ER -