Ly-49 receptor expression and functional analysis in multiple mouse strains

John R. Ortaldo, Anna T. Mason, Robin Winkler-Pickett, Arati Raziuddin, William J Murphy, Llewellyn H. Mason

Research output: Contribution to journalArticle

48 Scopus citations

Abstract

We present data on the strain distribution and functional characteristics of the Ly-49 receptors A, C/I, D, and G2 on DX5+ natural killer (NK) cells. We have examined tyrosine phosphorylation of the Ly-49 molecules, regulation of NK cytotoxic functions, and in vivo marrow rejection capability. The flow cytometry results demonstrate a diverse and complex pattern of expression of the Ly-49 receptors in the 11 strains examined. The vast majority of NK cells express Ly-49s, although some NK1.1+ CD3+ cells also express these receptors. The results of our functional analysis indicate that H-2D(d) was able to inhibit the function of Ly-49G2+ NK cells, not only in B6 mice, but also by NK cells derived from several haplotypes. The examination of Ly-49 receptor tyrosine phosphorylation, which is a biochemical measure of inhibitory function, was consistently observed in the 11 mouse strains examined. In contrast, analysis of Ly-49D function suggests its expression appears to be more restricted and that H-2D(d) is an activating ligand for this receptor. In addition, the in vivo examination of both inhibitory (Ly-49G2) and activating (Ly49D) receptors demonstrated regulatory roles of these class I binding receptors in marrow transplantation.

Original languageEnglish (US)
Pages (from-to)512-520
Number of pages9
JournalJournal of Leukocyte Biology
Volume66
Issue number3
StatePublished - 1999
Externally publishedYes

Keywords

  • Flow cytometry analysis
  • Ly-49
  • Natural killer
  • Tyrosine phosphorylation

ASJC Scopus subject areas

  • Cell Biology

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  • Cite this

    Ortaldo, J. R., Mason, A. T., Winkler-Pickett, R., Raziuddin, A., Murphy, W. J., & Mason, L. H. (1999). Ly-49 receptor expression and functional analysis in multiple mouse strains. Journal of Leukocyte Biology, 66(3), 512-520.