Lung vitamin E transport processes are affected by both age and environmental oxidants in mice

Giuseppe Valacchi, Vihas T. Vasu, Wallace Yokohama, Ana M. Corbacho, Anh Phung, Yunsook Lim, Hnin Hnin Aung, Carroll E Cross, Paul A. Davis

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Despite the physiological importance of alpha-tocopherol (AT), the molecular mechanisms involved in maintaining cellular and tissue tocopherol levels remain to be fully characterized. Scavenger receptor B1 (SRB1), one of a large family of scavenger receptors, has been shown to facilitate AT transfer from HDL to peripheral tissues via apo A-1-mediated processes and to be important in the delivery of AT to the lung cells. In the present studies the effects of age and two environmental oxidants ozone (O3) (0.25 ppm 6 h/day) and cigarette smoke (CS) (60 mg/m3 6 h/day) for 4 days on selected aspects of AT transport in murine lung tissues were assessed. While AT levels were 25% higher (p < 0.05) and 15% lower (p < 0.05) in plasma and lung tissue, respectively, in aged versus young mice, acute environmental exposure to O3 or CS at the doses used had no effect. Gene expression levels, determined by RT-PCR of AT transport protein (ATTP), SRB1, CD36, ATP binding cassette 3 (ABCA3) and ABCA1 and protein levels, determined by Western blots for SRB1, ATTP and ABCA1 were assessed. Aged mouse lung showed a lower levels of ATTP, ABCA3 and SRB1 and a higher level CD36 and ABCA1. Acute exposure to either O3 or CS induced declines in ATTP and SRB1 in both aged and young mice lung. CD36 increased in both young and aged mice lung upon exposure to O3 and CS. These findings suggest that both age and environmental oxidant exposure affect pathways related to lung AT homeostasis and do so in a way that favors declines in lung AT. However, given the approach taken, the effects cannot be traced to changes in these pathways or AT content in any specific lung associated cell type and thus highlight the need for further follow-up studies looking at specific lung associated cell types.

Original languageEnglish (US)
Pages (from-to)227-234
Number of pages8
JournalToxicology and Applied Pharmacology
Volume222
Issue number2
DOIs
StatePublished - Jul 15 2007

Fingerprint

alpha-Tocopherol
Vitamin E
Oxidants
Scavenger Receptors
Lung
Smoke
Tobacco Products
Carrier Proteins
Tissue
Environmental Exposure
ATP Binding Cassette Transporter 1
Adenosine Triphosphate
Tocopherols
Ozone
Apolipoprotein A-I
Gene expression
Protein Binding
Homeostasis
Western Blotting
Plasmas

Keywords

  • Aging
  • Cigarette smoke
  • Lung
  • Ozone
  • Vitamin E

ASJC Scopus subject areas

  • Pharmacology
  • Toxicology

Cite this

Valacchi, G., Vasu, V. T., Yokohama, W., Corbacho, A. M., Phung, A., Lim, Y., ... Davis, P. A. (2007). Lung vitamin E transport processes are affected by both age and environmental oxidants in mice. Toxicology and Applied Pharmacology, 222(2), 227-234. https://doi.org/10.1016/j.taap.2007.04.010

Lung vitamin E transport processes are affected by both age and environmental oxidants in mice. / Valacchi, Giuseppe; Vasu, Vihas T.; Yokohama, Wallace; Corbacho, Ana M.; Phung, Anh; Lim, Yunsook; Aung, Hnin Hnin; Cross, Carroll E; Davis, Paul A.

In: Toxicology and Applied Pharmacology, Vol. 222, No. 2, 15.07.2007, p. 227-234.

Research output: Contribution to journalArticle

Valacchi, G, Vasu, VT, Yokohama, W, Corbacho, AM, Phung, A, Lim, Y, Aung, HH, Cross, CE & Davis, PA 2007, 'Lung vitamin E transport processes are affected by both age and environmental oxidants in mice', Toxicology and Applied Pharmacology, vol. 222, no. 2, pp. 227-234. https://doi.org/10.1016/j.taap.2007.04.010
Valacchi, Giuseppe ; Vasu, Vihas T. ; Yokohama, Wallace ; Corbacho, Ana M. ; Phung, Anh ; Lim, Yunsook ; Aung, Hnin Hnin ; Cross, Carroll E ; Davis, Paul A. / Lung vitamin E transport processes are affected by both age and environmental oxidants in mice. In: Toxicology and Applied Pharmacology. 2007 ; Vol. 222, No. 2. pp. 227-234.
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