Luminescent lanthanide nanoparticles as labels in DNA microarrays for quantification of methyl tertiary butyl ether degrading bacteria

Ahjeong Son, Mikaela Nichkova, Dosi Dosev, Ian M. Kennedy, Krassimira R. Hristova

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

We report application of lanthanide nanoparticles for DNA quantification in a microarray platform as a substitute for conventional organic fluorophores. A non-PCR based DNA microarray assay for quantifying bacteria capable of biodegrading methyl tertiary-butyl ether (MTBE) was demonstrated. Probe DNA was immobilized on a glass surface, hybridized with biotinylated target DNA and subsequently incubated with Neutravidin-biofunctionalized nanoparticles. The fluorescence spot intensities, measured by a commercial laser scanner, show a linear relationship (R 2 = 0.98) with bacterial 16S rDNA over a range of target DNA concentrations, while the background fluo-rescence remained low. In addition, nanoparticles fluorescence shows a stronger intensity than Quasar570 (Cy3). Present sensitivity of the assay is 10 pM of target DNA. The selectivity of the DNA-nanoparticle-probes to discriminate a non-target DNA with two base pairs mismatch in the 16S rDNA gene sequence was shown. The use of Eu:Gd 2O 3 nanoparticles as biolabels provides a relatively non-toxic, inexpensive, rapid and sensitive alternative to the materials currently used in DNA microarrays.

Original languageEnglish (US)
Pages (from-to)2462-2467
Number of pages6
JournalJournal of Nanoscience and Nanotechnology
Volume8
Issue number5
StatePublished - May 2008

Keywords

  • DNA microarrays
  • Lanthanide europium oxide
  • Methylibium petroleiphilum PM1
  • MTBE
  • Nanoparticles

ASJC Scopus subject areas

  • Chemistry(all)
  • Materials Science(all)
  • Materials Science (miscellaneous)
  • Engineering (miscellaneous)

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