Luminal stability of insulin-like growth factors I and II in developing rat gastrointestinal tract

R. K. Rao, Anthony F Philipps, C. S. Williams, D. M. McCracken, O. Koldovsky

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Background: Insulin-like growth factors (IGF)-I and -II are present in milk of a number of mammalian species. The stability of IGF-I and -II in the intestinal lumen was investigated by measuring the proteolytic degradation of 125I-labeled IGF-I and IGF-II by rat (suckling and adult) intestinal luminal flushings in vitro. Methods: Degradation of 125I-labeled IGF-I and IGF-II was assessed by measuring the generation of acid-soluble radioactivity and the reduction of the amounts of peak activity (gel filtration). Degradation was confirmed by measuring the loss of immunoreactivity and receptor activity. Results: Incubation of 125I-IGF-I with midjejunal luminal flushings from 12-day-old suckling rats generated acid-soluble radioactivity in a time- and dose-(flushing) dependent manner, whereas incubation of 125I-IGF-II produced only minor amounts of acid-soluble radioactivity. Degradation activity in luminal flushings from adult rat intestine was several times greater than that in luminaL flushings from suckling rats. Degradation of 125I-IGF-II was several times lower than that of 125I-IGF-I in the intestinal luminal flushings from suckling and adult rats. The rate of decrease in immunoprecipitable 125I-IGF-I was considerably lower than the rate of decrease in receptor-active radioactivity. Except for immunoreactivity, analyses of acid-precipitable, peak-A, and receptor-active radioactivities demonstrate that IGF-II is relatively more stable than IGF-I in luminal flushings of suckling rat duodenum, jejunum, and midjejunum. Conclusions: These results show that the stability of IGF in the gastrointestinal lumen depends on the age of the animal and the segment of the gastrointestinal tract, as well as on the peptide isoform.

Original languageEnglish (US)
Pages (from-to)179-185
Number of pages7
JournalJournal of Pediatric Gastroenterology and Nutrition
Volume26
Issue number2
DOIs
StatePublished - Feb 1998
Externally publishedYes

Fingerprint

insulin-like growth factor II
Insulin-Like Growth Factor II
insulin-like growth factor I
Insulin-Like Growth Factor I
gastrointestinal system
Gastrointestinal Tract
suckling
Radioactivity
rats
degradation
Acids
acids
receptors
animal age
somatomedins
Somatomedins
Jejunum
jejunum
duodenum
Duodenum

Keywords

  • Metabolism
  • Peptidase
  • Peptides
  • Receptors
  • Small intestine
  • Stomach

ASJC Scopus subject areas

  • Gastroenterology
  • Histology
  • Medicine (miscellaneous)
  • Food Science
  • Pediatrics, Perinatology, and Child Health

Cite this

Luminal stability of insulin-like growth factors I and II in developing rat gastrointestinal tract. / Rao, R. K.; Philipps, Anthony F; Williams, C. S.; McCracken, D. M.; Koldovsky, O.

In: Journal of Pediatric Gastroenterology and Nutrition, Vol. 26, No. 2, 02.1998, p. 179-185.

Research output: Contribution to journalArticle

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abstract = "Background: Insulin-like growth factors (IGF)-I and -II are present in milk of a number of mammalian species. The stability of IGF-I and -II in the intestinal lumen was investigated by measuring the proteolytic degradation of 125I-labeled IGF-I and IGF-II by rat (suckling and adult) intestinal luminal flushings in vitro. Methods: Degradation of 125I-labeled IGF-I and IGF-II was assessed by measuring the generation of acid-soluble radioactivity and the reduction of the amounts of peak activity (gel filtration). Degradation was confirmed by measuring the loss of immunoreactivity and receptor activity. Results: Incubation of 125I-IGF-I with midjejunal luminal flushings from 12-day-old suckling rats generated acid-soluble radioactivity in a time- and dose-(flushing) dependent manner, whereas incubation of 125I-IGF-II produced only minor amounts of acid-soluble radioactivity. Degradation activity in luminal flushings from adult rat intestine was several times greater than that in luminaL flushings from suckling rats. Degradation of 125I-IGF-II was several times lower than that of 125I-IGF-I in the intestinal luminal flushings from suckling and adult rats. The rate of decrease in immunoprecipitable 125I-IGF-I was considerably lower than the rate of decrease in receptor-active radioactivity. Except for immunoreactivity, analyses of acid-precipitable, peak-A, and receptor-active radioactivities demonstrate that IGF-II is relatively more stable than IGF-I in luminal flushings of suckling rat duodenum, jejunum, and midjejunum. Conclusions: These results show that the stability of IGF in the gastrointestinal lumen depends on the age of the animal and the segment of the gastrointestinal tract, as well as on the peptide isoform.",
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T1 - Luminal stability of insulin-like growth factors I and II in developing rat gastrointestinal tract

AU - Rao, R. K.

AU - Philipps, Anthony F

AU - Williams, C. S.

AU - McCracken, D. M.

AU - Koldovsky, O.

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N2 - Background: Insulin-like growth factors (IGF)-I and -II are present in milk of a number of mammalian species. The stability of IGF-I and -II in the intestinal lumen was investigated by measuring the proteolytic degradation of 125I-labeled IGF-I and IGF-II by rat (suckling and adult) intestinal luminal flushings in vitro. Methods: Degradation of 125I-labeled IGF-I and IGF-II was assessed by measuring the generation of acid-soluble radioactivity and the reduction of the amounts of peak activity (gel filtration). Degradation was confirmed by measuring the loss of immunoreactivity and receptor activity. Results: Incubation of 125I-IGF-I with midjejunal luminal flushings from 12-day-old suckling rats generated acid-soluble radioactivity in a time- and dose-(flushing) dependent manner, whereas incubation of 125I-IGF-II produced only minor amounts of acid-soluble radioactivity. Degradation activity in luminal flushings from adult rat intestine was several times greater than that in luminaL flushings from suckling rats. Degradation of 125I-IGF-II was several times lower than that of 125I-IGF-I in the intestinal luminal flushings from suckling and adult rats. The rate of decrease in immunoprecipitable 125I-IGF-I was considerably lower than the rate of decrease in receptor-active radioactivity. Except for immunoreactivity, analyses of acid-precipitable, peak-A, and receptor-active radioactivities demonstrate that IGF-II is relatively more stable than IGF-I in luminal flushings of suckling rat duodenum, jejunum, and midjejunum. Conclusions: These results show that the stability of IGF in the gastrointestinal lumen depends on the age of the animal and the segment of the gastrointestinal tract, as well as on the peptide isoform.

AB - Background: Insulin-like growth factors (IGF)-I and -II are present in milk of a number of mammalian species. The stability of IGF-I and -II in the intestinal lumen was investigated by measuring the proteolytic degradation of 125I-labeled IGF-I and IGF-II by rat (suckling and adult) intestinal luminal flushings in vitro. Methods: Degradation of 125I-labeled IGF-I and IGF-II was assessed by measuring the generation of acid-soluble radioactivity and the reduction of the amounts of peak activity (gel filtration). Degradation was confirmed by measuring the loss of immunoreactivity and receptor activity. Results: Incubation of 125I-IGF-I with midjejunal luminal flushings from 12-day-old suckling rats generated acid-soluble radioactivity in a time- and dose-(flushing) dependent manner, whereas incubation of 125I-IGF-II produced only minor amounts of acid-soluble radioactivity. Degradation activity in luminal flushings from adult rat intestine was several times greater than that in luminaL flushings from suckling rats. Degradation of 125I-IGF-II was several times lower than that of 125I-IGF-I in the intestinal luminal flushings from suckling and adult rats. The rate of decrease in immunoprecipitable 125I-IGF-I was considerably lower than the rate of decrease in receptor-active radioactivity. Except for immunoreactivity, analyses of acid-precipitable, peak-A, and receptor-active radioactivities demonstrate that IGF-II is relatively more stable than IGF-I in luminal flushings of suckling rat duodenum, jejunum, and midjejunum. Conclusions: These results show that the stability of IGF in the gastrointestinal lumen depends on the age of the animal and the segment of the gastrointestinal tract, as well as on the peptide isoform.

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