LP-BM5 virus-infected mice produce activating autoantibodies to the AMPA receptor

Elena Koustova, Yoshitatsu Sei, Linda Fossom, Mei Ling Wei, Peter N R Usherwood, N. Bradley Keele, Michael A Rogawski, Anthony S. Basile

Research output: Contribution to journalArticlepeer-review

20 Scopus citations


Autoantibodies to α-amino-3-hydroxy-5-methylisoxazole-4-propionic acid (AMPA) receptors may contribute to chronic hyperexcitability syndromes and neurodegeneration, but their origin is unclear. We examined LP-BM5 murine leukemia virus-infected mice, which manifest excitotoxic brain lesions and hypergammaglobulinemia, for the presence of AMPA-receptor Ab's. Endogenous IgG accumulated upon neurons in the neocortex and caudate/putamen of infected mice and interacted with native and recombinant AMPA-receptor subunits with the following relative abundance: GluR3 ≥ GluR1 > GluR2 = GluR4, as determined by immunoprecipitation. In a radioligand assay, IgG preparations from infected mice specifically inhibited [3H]AMPA binding to receptors in brain homogenates, an activity that was lost after preadsorbing the IgG preparation to immobilized LP-BM5 virus. These IgGs also evoked currents when applied to hippocampal pyramidal neurons or to damaged cerebellar granule neurons. These currents could be blocked using any of several AMPA receptor antagonists. Thus, anti-AMPA-receptor Ab's can be produced as the result of a virus infection, in part through molecular mimicry. These Ab's may alter neuronal signaling and contribute to the neurodegeneration observed in these mice, actions that may be curtailed by the use of AMPA-receptor antagonists.

Original languageEnglish (US)
Pages (from-to)737-744
Number of pages8
JournalJournal of Clinical Investigation
Issue number6
StatePublished - 2001
Externally publishedYes

ASJC Scopus subject areas

  • Medicine(all)


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